Department of Biology, Faculty of Science and Letters, Mustafa Kemal University, Hatay, Turkey.
Environ Toxicol. 2014 Jun;29(6):631-41. doi: 10.1002/tox.21790. Epub 2012 Jun 22.
Thiacloprid, a neonicotinoid insecticide, is widely used for controlling various species of pests on many crops. The potential genotoxic effects of thiacloprid on human peripheral blood lymphocytes (PBLs) were investigated in vitro by the chromosome aberrations (CAs), sister chromatid exchanges (SCEs), and cytokinesis-block micronucleus (MN) assays. The human PBLs were treated with 75, 150, and 300 μg/mL thiacloprid in the absence and presence of an exogenous metabolic activator (S9 mix). Thiacloprid increased the CAs and SCEs significantly at all concentrations (75, 150, and 300 μg/mL) both in the absence and presence of the S9 mix and induced a significant increase in MN and nucleoplasmic bridge formations at all concentrations for 24 h and at 75 and 150 μg/mL for 48-h treatment periods in the absence of the S9 mix; and at all concentrations in the presence of the S9 mix when compared with the control and solvent control. Thiacloprid was also found to significantly induce nuclear bud (NBUD) formation at 300 μg/mL for 24 h and at 150 μg/mL for 48-h treatment times in the absence of the S9 mix and at the two highest concentrations (150 and 300 μg/mL) in the presence of the S9 mix. Thiacloprid significantly decreased the mitotic index, proliferation index, and nuclear division index for all concentrations both in the absence and presence of the S9 mix.
噻虫啉是一种新烟碱类杀虫剂，广泛用于防治多种作物上的各种害虫。本研究采用染色体畸变（CA）、姐妹染色单体交换（SCE）和胞质分裂微核（MN）试验，体外研究噻虫啉对人外周血淋巴细胞（PBL）的潜在遗传毒性。用噻虫啉（75、150 和 300μg/ml）在无和有外源性代谢激活剂（S9 混合液）条件下处理人 PBL。噻虫啉在无和有 S9 混合液的情况下，各浓度（75、150 和 300μg/ml）均明显增加 CA 和 SCE；无 S9 混合液时，MN 和核质桥形成显著增加，24 h 各浓度，48 h 时 75 和 150μg/ml 浓度；有 S9 混合液时，各浓度均显著增加。噻虫啉还显著增加 NBUD 形成，无 S9 混合液时 24 h 时 300μg/ml，48 h 时 150μg/ml；有 S9 混合液时，两个最高浓度（150 和 300μg/ml）也显著增加。无和有 S9 混合液时，噻虫啉各浓度均显著降低有丝分裂指数、增殖指数和核分裂指数。
