University of Turin, Department of Life Sciences and Systems Biology, Via Accademia Albertina n. 13, 10123, Torino, Italy.
Environ Sci Pollut Res Int. 2018 Dec;25(34):34693-34700. doi: 10.1007/s11356-018-3417-9. Epub 2018 Oct 15.
Glyphosate is an important broad-spectrum herbicide used in agriculture and residential areas for weed and vegetation control, respectively. In our study, we analyzed the in vitro clastogenic and/or aneugenic effects of glyphosate by chromosomal aberrations and micronuclei assays. Human lymphocytes were exposed to five glyphosate concentrations: 0.500, 0.100, 0.050, 0.025, and 0.0125 μg/mL, where 0.500 μg/mL represents the established acceptable daily intake value, and the other concentrations were tested in order to establish the genotoxicity threshold for this compound. We observed that chromosomal aberration (CA) and micronuclei (MNi) frequencies significantly increased at all tested concentrations, with exception of 0.0125 μg/mL. Vice versa, no effect has been observed on the frequencies of nuclear buds and nucleoplasmic bridges, with the only exception of 0.500 μg/mL of glyphosate that was found to increase in a significant manner the frequency of nucleoplasmic bridges. Finally, the cytokinesis-block proliferation index and the mitotic index were not significantly reduced, indicating that glyphosate does not produce effects on the proliferation/mitotic index at the tested concentrations.
草甘膦是一种重要的广谱除草剂，分别用于农业和住宅区的杂草和植被控制。在我们的研究中，我们通过染色体畸变和微核试验分析了草甘膦的体外致裂和/或非整倍性效应。我们将人类淋巴细胞暴露于五种草甘膦浓度下：0.500、0.100、0.050、0.025 和 0.0125μg/mL，其中 0.500μg/mL 代表已建立的可接受日摄入量值，而其他浓度则用于确定该化合物的遗传毒性阈值。我们观察到，在所有测试浓度下，染色体畸变（CA）和微核（MNi）的频率均显著增加，除了 0.0125μg/mL 外。相反，核芽和核质桥的频率没有观察到任何影响，唯一的例外是 0.500μg/mL 的草甘膦，其核质桥的频率显著增加。最后，细胞分裂阻断增殖指数和有丝分裂指数没有显著降低，表明草甘膦在测试浓度下不会对增殖/有丝分裂指数产生影响。
