Bayram Suleyman, Genc Ahmet, Buyukleyla Mehmet, Rencuzogullari Eyyup
Department of Nursing, Adiyaman School of Health, Adiyaman University, Adiyaman, Turkey.
Vocational School of Health Services, Adiyaman University, Adiyaman, Turkey.
Cytotechnology. 2016 Oct;68(5):2027-36. doi: 10.1007/s10616-016-9943-8. Epub 2016 Jan 11.
The genotoxicity of copper oxychloride was investigated in human lymphocytes using chromosome aberration (CA) and micronucleus (MN) tests and the randomly amplified polymorphic DNA-polymerase chain reaction technique. The lymphocytes were treated with 3, 6, and 12 µg/mL of copper oxychloride for 24 and 48 h. Copper oxychloride increased CA and abnormal cells in a dose-dependent manner. The frequency of MN and micronucleated binuclear cells also increased at all concentrations and treatment periods. However, copper oxychloride cytotoxicity, observed through lower mitotic and nuclear division index, was significantly lower only at the higher concentrations (6 and 12 µg/mL). Copper oxychloride increased the polymorphic bands and decreased genomic template stability. In conclusion, in this study it was confirmed that copper oxychloride has genotoxic potential for human lymphocytes in vitro. Additionally, caution is advised for its use as a fungicide, because it may increase the risk of exposure through the food chain.
使用染色体畸变（CA）试验、微核（MN）试验以及随机扩增多态性DNA - 聚合酶链反应技术，在人淋巴细胞中研究了碱式氯化铜的遗传毒性。将淋巴细胞用3、6和12μg/mL的碱式氯化铜处理24小时和48小时。碱式氯化铜以剂量依赖的方式增加了染色体畸变和异常细胞。在所有浓度和处理时间段，微核和微核双核细胞的频率也增加了。然而，通过较低的有丝分裂和核分裂指数观察到的碱式氯化铜细胞毒性，仅在较高浓度（6和12μg/mL）时显著降低。碱式氯化铜增加了多态性条带并降低了基因组模板稳定性。总之，在本研究中证实碱式氯化铜在体外对人淋巴细胞具有遗传毒性潜力。此外，由于其可能增加通过食物链暴露的风险，建议谨慎使用其作为杀菌剂。
