Adonina I G, Petrash N V, Timonova E M, Khristov Iu A, Salina E A
Genetika. 2012 Apr;48(4):488-94.
Genotyping was performed for the leaf rust-resistant line 73/00i (Triticum aestivum x Aegilops speltoides). Fluorescence in situ hybridization (FISH) with probes Spelt1 and pSc119.2 in combination with microsatellite analysis were used to determine the locations and sizes of the Ae. speltoides genetic fragments integrated into the line genome. Translocations were identified in the long arms of chromosomes 5B and 6B and in the short arm of chromosome 1B. The Spelt1 and pSc119.2 molecular cytological markers made it possible to rapidly establish lines with single translocation in the long arms of chromosomes 5B and 6B. The line carrying the T5BS x 5BL-5SL translocation was highly resistant to leaf rust, and the lines carrying the T6BS x 6BL-6SL translocation displayed moderate resistance. The translocations differed in chromosomal location from known leaf resistance genes transferred into common wheat from Ae. speltoides. Hence, it was assumed that new genes were introduced into the common wheat genome from Ae. speltoides. The locus that determined high resistance to leaf rust and was transferred into the common wheat genome from the long arm of Ae. speltoides chromosome 5S by the T5BS x 5BL-5SL translocation was preliminarily designated as LrAsp5.
对叶锈病抗性品系73/00i（普通小麦×斯卑尔脱山羊草）进行了基因分型。使用探针Spelt1和pSc119.2的荧光原位杂交（FISH）结合微卫星分析来确定整合到该品系基因组中的斯卑尔脱山羊草遗传片段的位置和大小。在5B和6B染色体的长臂以及1B染色体的短臂中鉴定出了易位。Spelt1和pSc119.2分子细胞学标记使得能够快速建立在5B和6B染色体长臂中具有单易位的品系。携带T5BS×5BL-5SL易位的品系对叶锈病高度抗性，而携带T6BS×6BL-6SL易位的品系表现出中度抗性。这些易位在染色体位置上与从斯卑尔脱山羊草转移到普通小麦中的已知叶抗性基因不同。因此，推测从斯卑尔脱山羊草向普通小麦基因组中引入了新基因。通过T5BS×5BL-5SL易位从斯卑尔脱山羊草5S染色体长臂转移到普通小麦基因组中并决定对叶锈病高抗性的位点初步命名为LrAsp5。
