Phipps D C, West J, Eby R, Koster M, Madore D V, Quataert S A
Department of Immunobiologic Laboratory Service, Praxis Biologics, Inc., Rochester, NY 14623.
J Immunol Methods. 1990 Dec 31;135(1-2):121-8. doi: 10.1016/0022-1759(90)90264-v.
An ELISA measuring total Ig antibodies to the capsular polysaccharide of Haemophilus influenzae type b (HbPs) in human sera using an antigen composed of Haemophilus b oligosaccharides conjugated to human serum albumin (HbO-HA) was shown to have an excellent correlation to the radioantigen binding assay (RABA). When 214 sera with different anti-HbPs levels were assayed for total Ig by HbO-HA ELISA and by RABA the correlation coefficient was 0.917 and the paired t test p value was 0.575. Use of competitive ELISA employing soluble HbPs, HbO-HA and human albumin as competitors, showed that the HbO-HA ELISA was specific for antibodies to HbPs. The HbO-HA ELISA yielded reproducible results both within and between laboratories. The HbO-HA ELISA can also be used to determine the isotype of anti-HbPs antibodies by using isotype specific enzyme conjugates. The sum of the IgG, IgA and IgM HbO-HA ELISA results had excellent correlation to the RABA results (correlation coefficient = 0.976). Thus, the HbO-HA ELISA can be substituted for the classical RABA and also be utilized for quantitating the isotype of the anti-HbPs antibodies.
一种采用与人血清白蛋白偶联的b型流感嗜血杆菌寡糖（HbO-HA）作为抗原，检测人血清中针对b型流感嗜血杆菌荚膜多糖（HbPs）的总Ig抗体的酶联免疫吸附测定（ELISA），被证明与放射抗原结合试验（RABA）具有良好的相关性。当采用HbO-HA ELISA和RABA对214份具有不同抗HbPs水平的血清进行总Ig检测时，相关系数为0.917，配对t检验p值为0.575。使用可溶性HbPs、HbO-HA和人白蛋白作为竞争剂的竞争性ELISA表明，HbO-HA ELISA对HbPs抗体具有特异性。HbO-HA ELISA在实验室内和实验室间均能产生可重复的结果。通过使用同种型特异性酶结合物，HbO-HA ELISA还可用于确定抗HbPs抗体的同种型。IgG、IgA和IgM的HbO-HA ELISA结果总和与RABA结果具有良好的相关性（相关系数 = 0.976）。因此，HbO-HA ELISA可替代经典的RABA，也可用于定量抗HbPs抗体的同种型。
