Hazlewood M, Nusrat R, Kumararatne D S, Goodall M, Raykundalia C, Wang D G, Joyce H J, Milford-Ward A, Forte M, Pahor A
Department of Immunology, Medical School, Dudley Road Hospital, Birmingham, UK.
Clin Exp Immunol. 1993 Aug;93(2):157-64. doi: 10.1111/j.1365-2249.1993.tb07959.x.
Antibody levels specific for capsular polysaccharides of Streptococcus pneumoniae and Haemophilus influenzae type b (Hib) and to tetanus toxoid (TT), were measured in serum samples of 750 age-stratified subjects from the UK. The study subjects comprised healthy adult volunteers and hospitalized children undergoing elective surgery, excluding those with a history of infection or under investigation for immunological or haematological disorders. These antibody levels were calibrated by comparison with serum pool obtained from healthy adult volunteers, who were immunized with Hib polyribose-phosphate vaccine (Merieux). The data are intended to provide reference ranges to assist in the interpretation of specific antibody measurements in the clinical setting. Maternal IgG pneumococcal capsular polysaccharide (PCP) specific antibody levels, geometric-mean titre (GMT) 1/22, were lost by 6 months of age (GMT of 1/9). They remained low until 3-5 years (GMT of 1/20), and consisted principally of IgG1. Thereafter, IgG anti-PCP antibody titres increased steadily to adult levels (GMT of 1/275), of which 80% was IgG2. Anti-PCP antibody titres of the IgM isotype rose steadily from a GMT 1/21 (0-6 months) to 1/420 (3-5 years), a level which was maintained until adulthood. Anti-Hib antibody concentrations, determined by RABA, again demonstrated the decline in maternal antibody, from 0.18 micrograms/ml in the 0-6 month age cohort, to 0.09 microgram/ml between 6 and 12 months. Geometric-mean antibody concentrations remained below 0.2 micrograms/ml until 3-5 years, then increased with age, attaining the mean adult level of 1.02 micrograms/ml. Anti-TT antibody concentrations were measured in the same sera, by ELISA. Two peaks in anti-TT antibody levels were seen in children of 0.059 IU/ml and 0.166 IU/ml corresponding to the schedule of routine childhood immunization in the first year and at 5 years of age.
在来自英国的750名按年龄分层的受试者的血清样本中，检测了针对肺炎链球菌和b型流感嗜血杆菌（Hib）荚膜多糖以及破伤风类毒素（TT）的抗体水平。研究对象包括健康成年志愿者和接受择期手术的住院儿童，排除有感染史或正在接受免疫或血液系统疾病调查的患者。这些抗体水平通过与用Hib多聚核糖磷酸疫苗（Merieux）免疫的健康成年志愿者获得的血清池进行比较来校准。这些数据旨在提供参考范围，以协助在临床环境中解释特定抗体测量结果。母体抗肺炎球菌荚膜多糖（PCP）特异性抗体水平，几何平均滴度（GMT）为1/22，在6个月大时降至1/9（GMT）。它们在3至5岁之前一直较低（GMT为1/20），主要由IgG1组成。此后，IgG抗PCP抗体滴度稳步上升至成人水平（GMT为1/275），其中80%为IgG2。IgM同种型的抗PCP抗体滴度从GMT 1/21（0至6个月）稳步上升至1/420（3至5岁），并维持在该水平直至成年。通过RABA测定的抗Hib抗体浓度再次显示母体抗体下降，从0至6个月年龄组的0.18微克/毫升降至6至12个月之间的0.09微克/毫升。几何平均抗体浓度在3至5岁之前一直低于0.2微克/毫升，然后随年龄增加，达到成人平均水平1.02微克/毫升。通过ELISA在相同血清中测量抗TT抗体浓度。在儿童中观察到抗TT抗体水平有两个峰值，分别为0.059 IU/ml和0.166 IU/ml，对应于第一年和5岁时的常规儿童免疫接种时间表。
