采用聚乙二醇沉淀法结合F(ab')2抗C3 ELISA检测循环免疫复合物。
Detection of circulating immune complexes with polyethylene glycol precipitation F(ab')2 anti-C3 ELISA.
作者信息
Xu R J, Gu Y A
机构信息
Department of Clinical Biochemistry, 97th Hospital of PLA, Xuzhou, China.
出版信息
J Immunol Methods. 1990 Dec 31;135(1-2):225-31. doi: 10.1016/0022-1759(90)90276-2.
A polyethylene glycol (PEG) precipitation F(ab')2 anti-C3 ELISA for the detection of complement-fixing IgG circulating immune complexes (CIC) is described. For this assay, test sera were treated with 3.5% PEG and then measured with F(ab')2 anti-C3 ELISA. The lower detection limit was 4 micrograms/ml of heat aggregated human IgG (HAHG). Intra-assay coefficient of variation (CV) was 4.9-8.3%. High levels of CIC are found in the sera of patients with systemic lupus erythematosus (SLE), hepatitis B and stomach cancer.
本文描述了一种用于检测补体结合型IgG循环免疫复合物（CIC）的聚乙二醇（PEG）沉淀F(ab')2抗C3 ELISA法。在该检测中，将待测血清用3.5%的PEG处理，然后用F(ab')2抗C3 ELISA进行检测。最低检测限为4微克/毫升热聚集人IgG（HAHG）。批内变异系数（CV）为4.9 - 8.3%。系统性红斑狼疮（SLE）、乙型肝炎和胃癌患者的血清中发现了高水平的CIC。
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