Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL, USA.
Bone Marrow Transplant. 2013 Jan;48(1):85-93. doi: 10.1038/bmt.2012.104. Epub 2012 Jun 25.
We evaluated immune reconstitution in 58 adults who received hematopoietic SCTs from allogeneic siblings (allosib), matched unrelated donors (MUD) or cord blood (CB) at 90-day intervals for 1 year post transplant. CB recipients had a higher incidence of infections in the first 100 days compared with allosib and MUD recipients. The number of circulating T cells was lower in CB recipients compared with MUD recipients at 90 days and compared with allosib recipients at 180 days. Spectratype analysis of the TCR Vβ complementarity determining region 3 (CDR3) of patient lymphocytes revealed that the TCR repertoire remained poorly diversified even at 360 days in nearly all patients. In contrast, the number of circulating B cells was significantly elevated in CB recipients compared with allosib recipients throughout the first year post transplant and compared with MUD recipients at 9-12 months. Spectratype analysis of the B-cell receptor V(H) CDR3 showed that the B-cell repertoire was diversified in most patients by 90 days. CD5(pos) B cells from assayed CB recipients expressed intracellular IL-10 early post transplant. Our data suggest that B cells, in addition to T cells, may have a role in impaired immune responses in CB transplant patients.
我们评估了 58 位成年人的免疫重建情况，这些成年人在接受异基因兄弟姐妹（allosib）、匹配的无关供体（MUD）或脐带血（CB）造血干细胞移植后，在移植后 1 年内每隔 90 天进行一次随访。CB 受者在前 100 天的感染发生率高于 alloSib 和 MUD 受者。与 MUD 受者相比，CB 受者在 90 天时循环 T 细胞数量较低，在 180 天时循环 T 细胞数量与 alloSib 受者相比也较低。患者淋巴细胞 TCR Vβ 互补决定区 3（CDR3）的谱型分析表明，TCR 受体库在几乎所有患者中甚至在 360 天时仍然多样性较差。相比之下，在移植后 1 年内，CB 受者的循环 B 细胞数量明显高于 alloSib 受者，在 9-12 个月时也高于 MUD 受者。B 细胞受体 V（H）CDR3 的谱型分析表明，在大多数患者中，B 细胞受体库在 90 天时就具有多样性。从检测到的 CB 受者中分离出的 CD5（pos）B 细胞在移植后早期表达细胞内 IL-10。我们的数据表明，B 细胞除了 T 细胞外，在 CB 移植患者的免疫应答受损中可能也有一定作用。
