苏云金芽孢杆菌纯化蛋白对重要农业害虫的特异性及功效

Specificity and efficacy of purified Bacillus thuringiensis proteins against agronomically important insects.

作者信息

MacIntosh S C, Stone T B, Sims S R, Hunst P L, Greenplate J T, Marrone P G, Perlak F J, Fischhoff D A, Fuchs R L

机构信息

Plant Science Technology, Monsanto Agricultural Company, St. Louis, Missouri 63198.

出版信息

J Invertebr Pathol. 1990 Sep;56(2):258-66. doi: 10.1016/0022-2011(90)90109-j.

DOI:10.1016/0022-2011(90)90109-j

PMID:2273290

Abstract

The host range and relative efficacy of three purified Bacillus thuringiensis insect control proteins were determined against 17 different agronomically important insects representing five orders and one species of mite. The three B. thuringiensis proteins were single gene products from B. thuringiensis ssp. kurstaki HD-1 (CryIA(b)) and HD-73 (CryIA(c)), both lepidopteran-specific proteins, and B. thuringiensis ssp. tenebrionis (CryIIIA), a coleopteran-specific protein. Seven insects showed sensitivity to both B. thuringiensis ssp. kurstaki proteins, whereas only 1 of the 18 insects was sensitive to B. thuringiensis ssp. tenebrionis protein. The level of B. thuringiensis ssp. kurstaki protein required for 50% mortality (LC50) varied by 2000-fold for these 7 insects. A larval growth inhibition assay was developed to determine the amount of B. thuringiensis ssp. kurstaki protein required to inhibit larval growth by 50% (EC50). This extremely sensitive assay enabled detection of B. thuringiensis ssp. kurstaki HD-73 levels as low as 1 ng/ml.

摘要

测定了三种纯化的苏云金芽孢杆菌杀虫蛋白对代表五个目和一种螨类的17种不同的具有重要农艺价值的昆虫的宿主范围和相对效力。这三种苏云金芽孢杆菌蛋白分别是来自苏云金芽孢杆菌库尔斯塔克亚种HD-1（CryIA(b)）和HD-73（CryIA(c)）的单基因产物，这两种都是鳞翅目特异性蛋白，以及苏云金芽孢杆菌暗黑变种（CryIIIA），一种鞘翅目特异性蛋白。七种昆虫对两种苏云金芽孢杆菌库尔斯塔克亚种蛋白均敏感，而在这18种昆虫中只有1种对苏云金芽孢杆菌暗黑变种蛋白敏感。对于这7种昆虫，导致50%死亡率（LC50）所需的苏云金芽孢杆菌库尔斯塔克亚种蛋白水平相差2000倍。开发了一种幼虫生长抑制试验来确定抑制幼虫生长50%（EC50）所需的苏云金芽孢杆菌库尔斯塔克亚种蛋白量。这种极其灵敏的试验能够检测到低至1纳克/毫升的苏云金芽孢杆菌库尔斯塔克亚种HD-73水平。