Department of Applied Biochemistry, Tokai University, 4-1-1 Kita-kaname, Hiratsuka-shi, Kanagawa 259-1292, Japan.
Glycoconj J. 2012 Oct;29(7):481-90. doi: 10.1007/s10719-012-9406-1. Epub 2012 Jun 26.
We evaluated the carbohydrate preferences of the C-type lectin receptors (CLRs) SIGNR1, SIGNR3, and Langerin as pathogen-uptake receptors based on uptake of liposomes consisting of cholesterol, DPPC, and various neoglycolipids at molar ratios of 10:10:1 and 10:7:4, respectively, using non-phagocytic CHO cells that express these receptors transiently. SIGNR1-expressing cells ingested liposomes coated with neoglycolipids with terminal mannose residues, such as Man2-, Man3-, and Man5-DPPE, and with a terminal N-acetylglucosamine. SIGNR1 mediated uptake of Man3-DPPE-coated liposomes most efficiently. Uptake of liposomes with lower neoglycolipid content by SIGNR3- or Langerin-expressing cells was slight or negligible, but uptake into these cells was detected for liposomes with higher neoglycolipid content. SIGNR1-expressing cells clearly ingested liposomes coated with Lewis X antigen, whereas SIGNR3- or Langerin-expressing cells barely ingested these liposomes, even at the higher neoglycolipid content. In contrast, SIGNR3 or Langerin, but not SIGNR1, mediated uptake of liposomes coated with blood group H antigen. These results indicate that CLRs with similar carbohydrate-recognition characteristics have distinct properties as pathogen-uptake receptors for carbohydrate-decorated particles.
我们评估了 C 型凝集素受体（CLRs）SIGNR1、SIGNR3 和 langerin 作为病原体摄取受体的碳水化合物偏好，这些受体基于胆固醇、DPPC 和各种糖基化脂的脂质体摄取，摩尔比分别为 10:10:1 和 10:7:4，使用瞬时表达这些受体的非吞噬性 CHO 细胞。表达 SIGNR1 的细胞摄取了末端甘露糖残基的糖基化脂，如 Man2-、Man3-和 Man5-DPPE，以及末端 N-乙酰葡萄糖胺修饰的脂质体。SIGNR1 介导 Man3-DPPE 修饰的脂质体摄取效率最高。SIGNR3 或 langerin 表达细胞对含有较低糖基化脂的脂质体摄取很少或可以忽略不计，但对含有较高糖基化脂的脂质体摄取则可检测到。表达 SIGNR1 的细胞明显摄取了路易斯 X 抗原修饰的脂质体，而表达 SIGNR3 或 langerin 的细胞几乎不摄取这些脂质体，即使是在较高的糖基化脂含量下也是如此。相比之下，SIGNR3 或 langerin 而非 SIGNR1 介导了富含血红细胞 H 抗原的脂质体的摄取。这些结果表明，具有相似碳水化合物识别特征的 CLRs 作为碳水化合物修饰颗粒的病原体摄取受体具有不同的特性。
