Department of Chemistry, Xiamen University, Xiamen, China.
J Sep Sci. 2012 Jun;35(10-11):1284-93. doi: 10.1002/jssc.201200115.
Lab on a chip (LOC) technology is a promising miniaturization approach. The feature that it significantly reduced sample consumption makes great sense in analytical and bioanalytical chemistry. Since the start of LOC technology, much attention has been focused on continuous flow microfluidic systems. At the turn of the century, droplet microfluidics, which was also termed segmented flow microfluidics, was introduced. Droplet microfluidics employs two immiscible phases to form discrete droplets, which are ideal vessels with confined volume, restricted dispersion, limited cross-contamination, and high surface area. Due to these unique features, droplet microfluidics proves to be a versatile tool in microscale sample handling. This article reviews the utility of droplet microfluidics in microanalytical systems with an emphasize on separation science, including sample encapsulation at ultra-small volume, compartmentalization of separation bands, isolation of droplet contents, and related detection techniques.
芯片实验室（LOC）技术是一种很有前途的微型化方法。其显著减少样品消耗的特点在分析和生物分析化学中具有重要意义。自 LOC 技术问世以来，人们一直关注连续流微流控系统。在本世纪之交，液滴微流控技术，也称为分段流微流控技术，被引入。液滴微流控技术采用两种不混溶的相形成离散的液滴，这些液滴是具有受限体积、限制扩散、有限交叉污染和高表面积的理想容器。由于这些独特的特点，液滴微流控技术在微尺度样品处理中证明是一种多功能工具。本文综述了液滴微流控技术在微分析系统中的应用，重点介绍了分离科学，包括超微量样品封装、分离带的分隔、液滴内容物的隔离以及相关检测技术。
