Itamura S, Iinuma H, Shida H, Morikawa Y, Nerome K, Oya A
Department of Virology and Rickettsiology, National Institute of Health, Tokyo, Japan.
J Gen Virol. 1990 Dec;71 ( Pt 12):2859-65. doi: 10.1099/0022-1317-71-12-2859.
Antibody and cytotoxic T lymphocyte (CTL) responses to the haemagglutinin (HA) of human H3N2 influenza virus were analysed, using recombinant vaccinia viruses containing the influenza HA gene inserted into the HA gene locus of vaccinia virus. The recombinant vaccinia viruses elicited a high haemagglutination inhibiting (HI) antibody response to the homologous influenza virus in mice. In addition, HI antibody generated by the recombinant vaccinia virus reacted with antigenic variants of human H3N2 influenza virus in a manner similar to that elicited by the HA vaccine. Mice with a high response to influenza virus HA vaccine were highly responsive to the HA expressed from the recombinant vaccinia virus, as measured by HI antibody production. The immunogenicity of the influenza virus HA expressed by the recombinant seems to be attributable to the intrinsic immunogenicity of the HA molecule. The recombinants primed mice for an influenza virus H3-specific CTL response and primed CTLs recognized the target cells in a subtype-specific manner. The results indicate that a recombinant vaccinia virus derived by the insertion of a foreign gene into its HA gene locus is a potent live vaccine not only for eliciting a high antibody response but also for priming a specific CTL response.
利用将流感血凝素（HA）基因插入痘苗病毒HA基因位点的重组痘苗病毒，分析了针对人H3N2流感病毒HA的抗体和细胞毒性T淋巴细胞（CTL）反应。重组痘苗病毒在小鼠中引发了针对同源流感病毒的高血凝抑制（HI）抗体反应。此外，重组痘苗病毒产生的HI抗体与人类H3N2流感病毒的抗原变异体发生反应，其方式与HA疫苗引发的反应相似。通过HI抗体产生来衡量，对流感病毒HA疫苗反应高的小鼠对重组痘苗病毒表达的HA也有高反应性。重组体表达的流感病毒HA的免疫原性似乎归因于HA分子的内在免疫原性。重组体使小鼠对流感病毒H3特异性CTL反应致敏，致敏的CTL以亚型特异性方式识别靶细胞。结果表明，通过将外源基因插入其HA基因位点而获得的重组痘苗病毒是一种有效的活疫苗，不仅可引发高抗体反应，还可使特异性CTL反应致敏。
