Yu Lin, Shi Jiandang, Cheng Sa, Zhu Yan, Zhao Xiulan, Yang Kuo, Du Xiaoling, Klocker Helmut, Yang Xiaoli, Zhang Ju
Departments of Biochemistry, Basic Medical College, Tianjin Medical University, Tianjin 300070, China.
Mol Endocrinol. 2012 Sep;26(9):1521-30. doi: 10.1210/me.2012-1006. Epub 2012 Jun 25.
As a key glycolytic enzyme, enolase 1 (ENO1) is critical for cellular energy metabolism. Recent studies have revealed its important role in growth and metastasis of lung, head and neck, and breast cancer. However, the regulatory mechanisms of ENO1 expression and secretion remain unclear. We observed that conditioned medium from estradiol-stimulated prostate stromal cells significantly promoted the migration of prostate cancer (PCa) cells. Two-dimensional protein electrophoresis, mass spectrometry, and immunodepletion assays identified one of the major active factors in the conditioned medium as α-type enolase (α-enolase, or ENO1). Moreover, in prostate stromal cells, estradiol not only enhanced the stability of ENO1 at the protein level in an estrogen receptor-α-dependent manner but also promoted its secretion to the extracellular matrix. Furthermore, recombinant ENO1 bound to the surface of PCa cells and promoted cell migration via their plasminogen receptor activity in a paracrine manner. Immunohistochemistry suggested that stromal ENO1 levels increased in PCa compared with those in normal tissue.
作为一种关键的糖酵解酶，烯醇化酶1（ENO1）对细胞能量代谢至关重要。最近的研究揭示了其在肺癌、头颈癌和乳腺癌的生长及转移中的重要作用。然而，ENO1表达和分泌的调控机制仍不清楚。我们观察到，来自雌二醇刺激的前列腺基质细胞的条件培养基显著促进了前列腺癌细胞（PCa）的迁移。二维蛋白质电泳、质谱分析和免疫去除试验确定条件培养基中的一种主要活性因子为α型烯醇化酶（α-烯醇化酶，即ENO1）。此外，在前列腺基质细胞中，雌二醇不仅以雌激素受体α依赖的方式在蛋白质水平增强ENO1的稳定性，还促进其分泌到细胞外基质中。此外，重组ENO1与PCa细胞表面结合，并通过其纤溶酶原受体活性以旁分泌方式促进细胞迁移。免疫组织化学表明，与正常组织相比，PCa中基质ENO1水平升高。
