Ou Guanghai, Xie Ruitao, Huang Jiansheng, Huang Jianpeng, Wen Zhenwei, Li Yu, Jiang Xintao, Ma Qian, Chen Gang
Fishery College, Guangdong Ocean University, Zhanjiang 524088, China.
Guangdong Evergreen Feed Industry Co., Ltd., Zhanjiang 524000, China.
Animals (Basel). 2023 Feb 28;13(5):887. doi: 10.3390/ani13050887.
We aimed to investigate the effects of dietary alpha-lipoic acid (α-LA) on the growth performance, serum biochemical indexes, liver morphology, antioxidant capacity, and transcriptome of juvenile hybrid groupers (♀ × ♂). Four experimental diets supplemented with 0 (SL0), 0.4 (L1), 0.6 (L2), and 1.2 (L3) g/kg α-LA were formulated and fed to three replicates of juvenile hybrid grouper (24.06 ± 0.15 g) for 56 d. The results indicated that dietary 0.4 and 0.6 g/kg α-LA significantly decreased the weight gain rate in juvenile hybrid groupers. Compared with SL0, the content of total protein in the serum of L1, L2, and L3 increased significantly, and alanine aminotransferase decreased significantly. The content of albumin in the serum of L3 increased significantly, and triglyceride, total cholesterol, and aspartate aminotransferase decreased significantly. In addition, the hepatocyte morphology in L1, L2, and L3 all showed varying degrees of improvement, and the activities of glutathione peroxidase and superoxide dismutase in the liver of L2 and L3 were significantly increased. A total of 42 differentially expressed genes were screened in the transcriptome data. KEGG showed that a total of 12 pathways were significantly enriched, including the pathway related to immune function and glucose homeostasis. The expression of genes (, , , and ) related to immune were significantly up-regulated, and the expressions of and genes related to glucose homeostasis were significantly down-regulated and up-regulated, respectively. In summary, dietary supplementation of 0.4 and 0.6 g/kg α-LA inhibited the growth performance of juvenile hybrid groupers. A total of 1.2 g/kg α-LA could reduce the blood lipid level, improve hepatocyte damage, and increase the hepatic antioxidant enzyme activity. Dietary α-LA significantly affected the pathway related to immune function and glucose homeostasis.
我们旨在研究日粮中α-硫辛酸（α-LA）对杂交石斑鱼幼鱼（♀×♂）生长性能、血清生化指标、肝脏形态、抗氧化能力和转录组的影响。配制了四种分别添加0（SL0）、0.4（L1）、0.6（L2）和1.2（L3）g/kg α-LA的实验饲料，投喂给杂交石斑鱼幼鱼（24.06±0.15 g），每组三个重复，为期56天。结果表明，日粮中添加0.4和0.6 g/kg α-LA显著降低了杂交石斑鱼幼鱼的增重率。与SL0组相比，L1、L2和L3组血清总蛋白含量显著升高，谷丙转氨酶显著降低。L3组血清白蛋白含量显著升高，甘油三酯、总胆固醇和谷草转氨酶显著降低。此外，L1、L2和L3组肝细胞形态均有不同程度改善，L2和L3组肝脏中谷胱甘肽过氧化物酶和超氧化物歧化酶活性显著升高。在转录组数据中总共筛选出42个差异表达基因。KEGG分析表明，共有12条通路显著富集，包括与免疫功能和葡萄糖稳态相关的通路。与免疫相关的基因（、、、和）表达显著上调，与葡萄糖稳态相关的基因和表达分别显著下调和上调。综上所述，日粮中添加0.4和0.6 g/kg α-LA抑制了杂交石斑鱼幼鱼的生长性能。添加1.2 g/kg α-LA可降低血脂水平，改善肝细胞损伤，并提高肝脏抗氧化酶活性。日粮α-LA显著影响与免疫功能和葡萄糖稳态相关的通路。
