Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China.
J Biomed Opt. 2012 Jun;17(6):066002. doi: 10.1117/1.JBO.17.6.066002.
We performed large area nonlinear optical microscopy (NOM) for label-free monitoring of the process of pulmonary melanoma metastasis ex vivo with subcellular resolution in C57BL/6 mice. Multiphoton autofluorescence (MAF) and second harmonic generation (SHG) images of lung tissue are obtained in a volume of ≈ 2.2 mm × 2.2 mm × 30 μm. Qualitative differences in morphologic features and quantitative measurement of pathological lung tissues at different time points are characterized. We find that combined with morphological features, the quantitative parameters, such as the intensity ratio of MAF and SHG between pathological tissue and normal tissue and the MAF to SHG index versus depth clearly shows the tissue physiological changes during the process of pulmonary melanoma metastasis. Our results demonstrate that large area NOM succeeds in monitoring the process of pulmonary melanoma metastasis, which can provide a powerful tool for the research in tumor pathophysiology and therapy evaluation.
我们使用大面积非线性光学显微镜（NOM）对 C57BL/6 小鼠的肺黑色素瘤转移过程进行了无标记的亚细胞分辨率监测。在 ≈ 2.2mm×2.2mm×30μm 的体积中获得了肺部组织的多光子自发荧光（MAF）和二次谐波产生（SHG）图像。对不同时间点的病理性肺组织的形态特征和定量测量进行了定性差异分析。我们发现，结合形态特征，定量参数，如病理性组织与正常组织之间的 MAF 和 SHG 的强度比以及 MAF 与 SHG 指数与深度的关系，清楚地显示了肺黑色素瘤转移过程中的组织生理变化。我们的结果表明，大面积 NOM 成功地监测了肺黑色素瘤转移的过程，为肿瘤病理生理学和治疗评估的研究提供了有力的工具。
