School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney 2052, Australia.
Mitochondrion. 2012 Jul;12(4):459-64. doi: 10.1016/j.mito.2012.06.005. Epub 2012 Jun 24.
The influence of mitochondrial DNA (mtDNA) mutations on human disease has been extensively studied, but the impact of mutations within the adaptive range is debated. We studied males from lines of Drosophila melanogaster that have a highly standardized nuclear genome but different mtDNA, at two ages. We measured mitochondrial respiration on permeabilized muscle fibers, hydrogen peroxide production of isolated mitochondria and mtDNA copy number of whole individuals. The results show that a small set of naturally occurring mtDNA mutations can have a significant influence on mitochondrial bioenergetics that may change as the organism ages.
线粒体 DNA（mtDNA）突变对人类疾病的影响已得到广泛研究，但突变在适应范围内的影响仍存在争议。我们研究了具有高度标准化核基因组但 mtDNA 不同的黑腹果蝇品系的雄性个体，分别在两个年龄段进行测量。我们测量了透化肌肉纤维的线粒体呼吸、分离线粒体的过氧化氢产生以及整个个体的 mtDNA 拷贝数。结果表明，一小部分自然发生的 mtDNA 突变可能对线粒体生物能量学产生重大影响，而这种影响可能会随着生物体的衰老而发生变化。
