Sahin Erinc, Roberts Christopher J
Drug Product Science & Technology R&D, Bristol-Myers Squibb, New Brunswick, NJ, USA.
Methods Mol Biol. 2012;899:403-23. doi: 10.1007/978-1-61779-921-1_25.
In this chapter, application of size exclusion chromatography with inline multi-angle light scattering (SEC-MALS) to protein systems is reviewed, in particular for its use in elucidating mechanistic details of net-irreversible aggregation processes. After motivating why SEC-MALS or analogous techniques are natural choices to interrogate such aggregating systems, the individual techniques (SEC and MALS) are reviewed briefly, as needed for the context of the remainder of the chapter. Illustrative examples are provided to highlight when and how SEC-MALS can be applied to test mass-action kinetic models for protein aggregation. Limitations of the technique, as well as recommendations for troubleshooting and potential errors in data interpretation are also provided.
在本章中，将综述体积排阻色谱联用在线多角度光散射技术（SEC-MALS）在蛋白质体系中的应用，特别是其在阐明净不可逆聚集过程的机理细节方面的应用。在阐述为何SEC-MALS或类似技术是研究此类聚集体系的自然选择之后，根据本章其余内容的上下文需要，简要回顾了各个技术（SEC和MALS）。提供了一些示例，以突出SEC-MALS何时以及如何应用于测试蛋白质聚集的质量作用动力学模型。还介绍了该技术的局限性，以及故障排除建议和数据解释中可能出现的错误。
