Department of Food Safety and Quality, Veterinary Academy, Lithuanian University of Health Sciences, Tilžės 18, 47181 Kaunas, Lithuania.
Curr Microbiol. 2012 Oct;65(4):398-406. doi: 10.1007/s00284-012-0170-z. Epub 2012 Jun 27.
A set of C. jejuni isolates of different origins and flaA-genotypes obtained throughout the broiler meat production chain was tested in this study for a possible correlation of their origin, phylogenetic relationship, and phenotypic properties. Interestingly, the results showed a correlation of the origin and the phylogenetic relationship between the C. jejuni isolates and their ability to form biofilm, but not in their ability to survive at -18, 5, 20, and 48 °C. Two strains, a broiler cloacae isolate and a broiler fillet isolate, were unable to develop biofilm, while most of the C. jejuni isolates originating from meat and surfaces of the slaughterhouse readily formed biofilms after both 24, 48, and 72 h. Interestingly, these biofilm-forming strains were closely related. Furthermore, two strains that were isolated after disinfection developed significantly more biofilms after 24 h of incubation than the remaining strains. A comparative genomic analysis using DNA microarrays showed that the gene contents of strains that efficiently formed biofilms were different from those that did not. The study suggests that biofilm formation might be a lineage specific property, allowing C. jejuni to both survive environmental stress at the slaughterhouse and to attach to the surface of meat.
本研究检测了一组来自不同起源和 flaA 基因型的 C. jejuni 分离株，以研究它们的起源、系统发育关系和表型特性之间是否存在相关性。有趣的是，结果表明 C. jejuni 分离株的起源和系统发育关系与其形成生物膜的能力相关，但与它们在-18、5、20 和 48°C 下的存活能力无关。有两株，即鸡泄殖腔分离株和鸡胸脯肉分离株，不能形成生物膜，而大多数源自肉类和屠宰场表面的 C. jejuni 分离株在 24、48 和 72 h 后均能很好地形成生物膜。有趣的是，这些形成生物膜的菌株密切相关。此外，在消毒后分离出的两株菌株在孵育 24 h 后形成的生物膜明显多于其余菌株。使用 DNA 微阵列进行的比较基因组分析表明，生物膜形成效率高的菌株的基因含量与不形成生物膜的菌株不同。该研究表明，生物膜的形成可能是一种谱系特异性特性，使 C. jejuni 既能在屠宰场的环境压力下存活，又能附着在肉类表面。
