Monteiro Guilherme Paz, Melo Roberta Torres de, Guidotti-Takeuchi Micaela, Dumont Carolyne Ferreira, Ribeiro Rosanne Aparecida Capanema, Guerra Wendell, Ramos Luana Munique Sousa, Paixão Drielly Aparecida, Santos Fernanda Aparecida Longato Dos, Rodrigues Dália Dos Prazeres, Boleij Peter, Hoepers Patrícia Giovana, Rossi Daise Aparecida
Laboratory of Molecular Epidemiology, Federal University of Uberlândia, Uberlândia 38402-018, Brazil.
Institute of Chemistry, Federal University of Uberlândia, Uberlândia 38402-018, Brazil.
Antibiotics (Basel). 2022 Mar 15;11(3):388. doi: 10.3390/antibiotics11030388.
spp. continues to figure prominently in world epidemiological registries as one of the leading causes of bacterial foodborne disease. We characterised 43 Brazilian lineages of Typhimurium (ST) strains, characterized drug resistance patterns, tested copper (II) complex as control options, and proposed effective antimicrobial measures. The minimum inhibitory concentration was evaluated for seven antimicrobials, isolated and combined with the copper (II) complex [Cu(4-FH)(phen)(ClO)] (4-FH = 4-fluorophenoxyacetic acid hydrazide and phen = 1,10-phenanthroline), known as DRI-12, in planktonic and sessile ST. In parallel, 42 resistance genes were screened (PCR/microarray). All strains were multidrug resistant (MDR). Resistance to carbapenems and polymyxins (86 and 88%, respectively) have drawn attention to the emergence of the problem in Brazil, and resistance is observed also to CIP and CFT (42 and 67%, respectively), the drugs of choice in treatment. Resistance to beta-lactams was associated with the genes / in 39% of the strains. Lower concentrations of DRI-12 (62.7 mg/L, or 100 μM) controlled planktonic and sessile ST in relation to AMP/SUL/TET and AMP/SUL/TET/COL, respectively. The synergistic effect provided by DRI-12 was significant for COL/CFT and COL/AMP in planktonic and sessile ST, respectively, and represents promising alternatives for the control of MDR ST.
鼠伤寒沙门氏菌（Salmonella Typhimurium，ST）作为细菌性食源性疾病的主要病因之一，在世界流行病学登记中仍然占据显著地位。我们对43株巴西鼠伤寒沙门氏菌菌株进行了特征分析，确定了其耐药模式，测试了铜（II）配合物作为控制方案，并提出了有效的抗菌措施。评估了七种抗菌药物对浮游态和固着态鼠伤寒沙门氏菌的最低抑菌浓度，这些抗菌药物单独使用以及与铜（II）配合物[Cu(4-FH)(phen)(ClO)]（4-FH = 4-氟苯氧基乙酸酰肼，phen = 1,10-菲啰啉）联合使用，该铜（II）配合物称为DRI-12。同时，筛选了42个耐药基因（采用聚合酶链反应/微阵列技术）。所有菌株均为多重耐药（MDR）。对碳青霉烯类和多粘菌素的耐药率（分别为86%和88%）引起了对巴西该问题出现的关注，同时也观察到对治疗中首选药物环丙沙星（CIP）和头孢噻呋（CFT）的耐药情况（分别为42%和67%）。39%的菌株中，对β-内酰胺类的耐药与某些基因有关。较低浓度的DRI-12（62.7 mg/L，即100 μM）分别相对于氨苄西林/磺胺甲恶唑/四环素（AMP/SUL/TET）和氨苄西林/磺胺甲恶唑/四环素/黏菌素（AMP/SUL/TET/COL）能控制浮游态和固着态的鼠伤寒沙门氏菌。DRI-12提供的协同效应分别在浮游态和固着态鼠伤寒沙门氏菌中对黏菌素/头孢噻呋（COL/CFT）和黏菌素/氨苄西林（COL/AMP）具有显著意义，代表了控制多重耐药鼠伤寒沙门氏菌的有前景的替代方案。
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