Li Hao-jie, Shen Zhen-bin, Sun Yi-hong, Liu Feng-lin, Wang Hong-shan, Chen Wei-dong
Department of General Surgery, Fudan University, Shanghai, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2012 Jun;15(6):618-21.
To investigate the association of the expressions of Th1 cytokines in gastric cancer tissue and the prognosis of patients with gastric cancer after radical resection.
Fifty-eight patients with gastric cancer treated at the Zhongshan Hospital of Fudan University were retrospectively analyzed. The expressions of Th1 cytokines mRNA were detected in tumor tissues by real-time polymerase chain reaction(RT-PCR) method in Th1 cells including TRAV10, IRF1, TBX21, CD3Z, GZMB, GATA3, and IFNG. Association of Th1 cytokines mRNA expressions and prognosis was evaluated.
The median follow up was 42.5(1-64) months. The 1-year survival rate was 84.5% and the 3-year survival rate was 72.4%. Univariate Cox regression analysis showed that lymph node metastasis, distant metastasis, TNM staging, lymphovascular invasion, GNLY mRNA expression, and the overall expression level of the 8 types of Th1 cells were associated with the prognosis of patients with gastric cancer(all P<0.05). The 3-year survival was 86.2% in patients with increased expression of mRNA and 58.6% in those with decreased expression. The 3-year survival was 79.6% in patients with any increase in the 8 Th1 cytokines and 33.3% in those with consistent downregulation of the 8 cytokines. Multivariate Cox analysis showed that lymph node metastasis and the overall expression level of 8 Th1 cytokines were independent risk factors associated with the prognosis of patients with gastric cancer in this cohort(both P<0.05).
The mRNA expression of GNLY is associated with the prognosis of patients with gastric cancer but is not an independent risk factor. The combination of mRNA expressions of the eight cytokines is an independent prognostic factor.
探讨胃癌组织中Th1细胞因子表达与胃癌根治术后患者预后的关系。
回顾性分析复旦大学附属中山医院收治的58例胃癌患者。采用实时聚合酶链反应（RT-PCR）法检测肿瘤组织中Th1细胞因子mRNA的表达，包括TRAV10、IRF1、TBX21、CD3Z、GZMB、GATA3和IFNG。评估Th1细胞因子mRNA表达与预后的关系。
中位随访时间为42.5（1-64）个月。1年生存率为84.5%，3年生存率为72.4%。单因素Cox回归分析显示，淋巴结转移、远处转移、TNM分期、淋巴管侵犯、GNLY mRNA表达以及8种Th1细胞的总体表达水平与胃癌患者的预后相关（均P<0.05）。mRNA表达增加的患者3年生存率为86.2%，表达降低的患者为58.6%。8种Th1细胞因子中任何一种增加的患者3年生存率为79.6%，8种细胞因子均下调的患者为33.3%。多因素Cox分析显示，淋巴结转移和8种Th1细胞因子的总体表达水平是该队列中与胃癌患者预后相关的独立危险因素（均P<0.05）。
GNLY的mRNA表达与胃癌患者的预后相关，但不是独立危险因素。8种细胞因子mRNA表达的组合是独立的预后因素。
