一种用于分析视网膜下假体的视网膜生理的新型体外传感配置。

A novel in vitro sensing configuration for retinal physiology analysis of a sub-retinal prosthesis.

机构信息

Inter-university Semiconductor Research Center, Automation System Research Institute, School of Electrical Engineering and Computer Science, Seoul National University, Seoul 151-744, Korea.

出版信息

Sensors (Basel). 2012;12(3):3131-44. doi: 10.3390/s120303131. Epub 2012 Mar 6.

DOI:10.3390/s120303131

PMID:22736997

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3376591/

Abstract

This paper presents a novel sensing configuration for retinal physiology analysis, using two microelectrode arrays (MEAs). In order to investigate an optimized stimulation protocol for a sub-retinal prosthesis, retinal photoreceptor cells are stimulated, and the response of retinal ganglion cells is recorded in an in vitro environment. For photoreceptor cell stimulation, a polyimide-substrate MEA is developed, using the microelectromechanical systems (MEMS) technology. For ganglion cell response recording, a conventional glass-substrate MEA is utilized. This new sensing configuration is used to record the response of retinal ganglion cells with respect to three different stimulation methods (monopolar, bipolar, and dual-monopolar stimulation methods). Results show that the geometrical relation between the stimulation microelectrode locations and the response locations seems very low. The threshold charges of the bipolar stimulation and the monopolar stimulation are in the range of 10~20 nC. The threshold charge of the dual-monopolar stimulation is not obvious. These results provide useful guidelines for developing a sub-retinal prosthesis.

摘要

本文提出了一种使用两个微电极阵列（MEA）进行视网膜生理分析的新型传感配置。为了研究用于视网膜假体的优化刺激方案，在体外环境中刺激视网膜光感受器细胞，并记录视网膜神经节细胞的反应。对于光感受器细胞刺激，使用微机电系统（MEMS）技术开发了聚酰亚胺衬底 MEA。对于神经节细胞反应记录，使用传统的玻璃衬底 MEA。这种新的传感配置用于记录视网膜神经节细胞对三种不同刺激方法（单极、双极和双单极刺激方法）的反应。结果表明，刺激微电极位置与响应位置之间的几何关系似乎非常低。双极刺激和单极刺激的阈值电荷在 10~20 nC 范围内。双单极刺激的阈值电荷不明显。这些结果为开发视网膜下假体提供了有用的指导。