Ai J, Ebrahimi S, Khoshzaban A, Jafarzadeh Kashi T S, Mehrabani D
Department of Tissue Engineering and Cell Therapy, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran.
Iran Red Crescent Med J. 2012 Feb;14(2):96-103. Epub 2012 Feb 1.
Bone healing and its reconstruction in fractures, especially in long bones are of particular importance in regenerative medicine. This study compares the bone healing rate after a human xenograft of mineralized bone and together with an allograft of bone marrow mesenchymal stem cells (MSCs) in an experimental tibial bone fracture rabbit model.
In fall 2009, twenty New Zealand white rabbits were randomly divided into 2 equal groups. In both groups, a 5 mm segmental defect was created in the right tibia. In group A, a scaffold pin was seeded with allogenic rabbit MSCs and was placed in the defect area and in group B, the defect was filled with an unseeded pin human mineralized bone xenograft. An untreated defect was induced in the left tibia of all animals serving as the control. After 4-8 weeks, the segmental defects were histologically evaluated and also by a compressive test.
In groups A and B, healing and formation of new bony tissue were significantly more than the control group and with a significant less inflammation.
Tissue engineering of mineralized bone xenograft and MSCs allograft may be significant steps in bone healing and regenerative medicine.
在再生医学中，骨折尤其是长骨骨折后的骨愈合及其重建尤为重要。本研究在实验性兔胫骨骨折模型中比较了人矿化骨异种移植以及与骨髓间充质干细胞（MSCs）同种异体移植后的骨愈合率。
2009年秋季，将20只新西兰白兔随机分为两组，每组数量相等。两组均在右侧胫骨制造一个5毫米的节段性缺损。A组将同种异体兔MSCs接种于支架针上并置于缺损区域，B组缺损处填充未接种细胞的人矿化骨异种移植针。所有动物的左侧胫骨制造未经处理的缺损作为对照。4至8周后，对节段性缺损进行组织学评估及压缩试验。
A组和B组新骨组织的愈合和形成均显著多于对照组，且炎症反应显著减轻。
矿化骨异种移植和MSCs同种异体移植的组织工程可能是骨愈合和再生医学中的重要步骤。
