Faramarzi Hossein, Mehrabani Davood, Fard Maryam, Akhavan Maryam, Zare Sona, Bakhshalizadeh Shabnam, Manafi Amir, Kazemnejad Somaieh, Shirazi Reza
Department of Infectious Diseases, Larestan School of Medical Sciensce, Larestan, Iran;
Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Science, Shiraz, Iran;; Department of Regenerative Medicine, University of Manitoba, Winnipeg, MB, Canada;
World J Plast Surg. 2016 Jan;5(1):26-31.
Menstrual blood-derived stem cells (MenSCs) are a novel source of stem cells that can be easily isolated non-invasively from female volunteered donor without ethical consideration. These mesenchymal-like stem cells have high rate of proliferation and possess multi lineage differentiation potency. This study was undertaken to isolate the MenSCs and assess their potential in differentiation into epidermal lineage.
About 5-10 ml of menstrual blood (MB) was collected using sterile Diva cups inserted into vagina during menstruation from volunteered healthy fertile women aged between 22-30 years. MB was transferred into Falcon tubes containing phosphate buffered saline (PBS) without Ca2(+) or Mg2(+) supplemented with 2.5 µg/ml fungizone, 100 µg/mL streptomycin, 100 U/mL penicillin and 0.5 mM EDTA. Mononuclear cells were separated using Ficoll-Hypaque density gradient centrifugation and washed out in PBS. The cell pellet was suspended in DMEM-F12 medium supplemented with 10% FBS and cultured in tissue culture plates. The isolated cells were co-cultured with keratinocytes derived from the foreskin of healthy newborn male aged 2-10 months who was a candidate for circumcision for differentiation into epidermal lineage.
The isolated MenSCs were adhered to the plate and exhibited spindle-shaped morphology. Flow cytometric analysis revealed the expression of mesenchymal markers of CD10, CD29, CD73, and CD105 and lack of hematopoietic stem cells markers. An early success in derivation of epidermal lineage from MenSCs was visible.
The MenSCs are a real source to design differentiation to epidermal cells that can be used non-invasively in various dermatological lesions and diseases.
月经血源性干细胞（MenSCs）是一种新型干细胞来源，可轻松地从女性自愿捐献者中无创分离，不存在伦理问题。这些间充质样干细胞具有高增殖率并具备多谱系分化潜能。本研究旨在分离MenSCs并评估其向表皮谱系分化的潜力。
在月经期间，使用无菌阴道收集杯从年龄在22 - 30岁的健康育龄自愿女性阴道中收集约5 - 10毫升月经血（MB）。将MB转移至含有无Ca2(+)或Mg2(+)的磷酸盐缓冲盐水（PBS）的 Falcon管中，添加2.5μg/ml两性霉素B、100μg/mL链霉素、100U/mL青霉素和0.5mM乙二胺四乙酸。使用Ficoll - Hypaque密度梯度离心法分离单核细胞，并在PBS中洗涤。将细胞沉淀悬浮于补充有10%胎牛血清的DMEM - F12培养基中，并在组织培养板中培养。将分离的细胞与来自2 - 10个月大健康新生儿包皮的角质形成细胞共培养，该新生儿为包皮环切术候选者，用于向表皮谱系分化。
分离的MenSCs贴壁生长并呈现纺锤形形态。流式细胞术分析显示表达间充质标志物CD10、CD29、CD73和CD105，且缺乏造血干细胞标志物。可见MenSCs向表皮谱系分化取得了初步成功。
MenSCs是设计分化为表皮细胞的真正来源，可无创用于各种皮肤病变和疾病。
