Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agriculture University, Hubei, 430070 Wuhan, China.
Mol Biol Rep. 2012 Nov;39(11):9903-9. doi: 10.1007/s11033-012-1857-8. Epub 2012 Jun 28.
Porcine reproductive and respiratory syndrome virus (PRRSV) could infect porcine alveolar macrophages (PAM), and the CD169 and CD163 are identified as critical receptors on the surface of PAM, but whether the single nucleotide polymorphisms (SNPs) of these genes could influence the infection is remain unclear. In this study, we identified totally 6 SNPs for CD169 (G1640T, C1654A, C4175T) and CD163 (G2277A, A2552G and C2700A), and evaluated their associations with PRRSV infection using two classified methods in a 524 pig population to investigate the effects of mutations on the PRRSV receptors. The pigs with genotypes of AA of CD169-C1654A, CT of CD169-C4175T and AA of CD163-A2552G appeared to resistant to the PRRSV infection by the combination of two classified results. The results provided fundamental molecular investigation to promote pig breeding with disease resistance. However, the identification of functional changes induced by SNPs and molecular mechanism were need further research.
猪繁殖与呼吸综合征病毒（PRRSV）可感染猪肺泡巨噬细胞（PAM），CD169 和 CD163 被鉴定为 PAM 表面的关键受体，但这些基因的单核苷酸多态性（SNP）是否会影响感染尚不清楚。在本研究中，我们总共鉴定了 CD169（G1640T、C1654A、C4175T）和 CD163（G2277A、A2552G 和 C2700A）的 6 个 SNP，并使用两种分类方法在 524 头猪群中评估了它们与 PRRSV 感染的相关性，以研究突变对 PRRSV 受体的影响。CD169-C1654A 的 AA 基因型、CD169-C4175T 的 CT 基因型和 CD163-A2552G 的 AA 基因型的猪似乎通过两种分类结果的组合对 PRRSV 感染具有抗性。该结果为促进抗病性猪种培育提供了基础的分子研究。然而，还需要进一步研究 SNP 引起的功能变化和分子机制。
