Kelly J M, Drysdale M R, Sealy-Lewis H M, Jones I G, Lockington R A
Department of Genetics, University of Adelaide, Australia.
Mol Gen Genet. 1990 Jul;222(2-3):323-8. doi: 10.1007/BF00633836.
An alcohol dehydrogenase was shown to be induced in Aspergillus nidulans by periods of anaerobic stress. This alcohol dehydrogenase was shown to correspond to the previously described cryptic enzyme, alcohol dehydrogenase III (McKnight et al. 1985), by analysis of a mutation in the structural gene of alcohol dehydrogenase III, alcC, created by gene disruption. Survival tests on agar plates showed that this enzyme is required for long-term survival under anaerobic conditions. Northern blot analysis and gene fusion studies showed that the expression of the alcC gene is regulated at both the transcriptional and translational levels. Thus there are mechanisms in this filamentous fungus allowing survival under anaerobic stress that are similar to those described in higher plants.
研究表明，在构巢曲霉中，厌氧胁迫期可诱导一种乙醇脱氢酶的产生。通过对乙醇脱氢酶III（alcC）结构基因中因基因破坏而产生的突变进行分析，发现这种乙醇脱氢酶与先前描述的隐性酶乙醇脱氢酶III（McKnight等人，1985年）相对应。琼脂平板上的存活试验表明，这种酶是厌氧条件下长期存活所必需的。Northern印迹分析和基因融合研究表明，alcC基因的表达在转录和翻译水平上均受到调控。因此，这种丝状真菌中存在与高等植物中描述的类似的在厌氧胁迫下存活的机制。
