Osada J, Aylagas H, Mirõ-Obradors M J, Arce C, Palacios-Alaiz E, Cascales M
Human Nutrition Research Center on Aging, Tufs University, Boston, MA 02111.
Neurochem Res. 1990 Sep;15(9):927-31. doi: 10.1007/BF00965914.
Brain phospholipid composition and the [32P]orthophosphate incorporation into brain phospholipids of control and rats treated for 3 days with thioacetamide were studied. Brain phospholipid content, phosphatidylcholine, phosphatidylethanolamine, lysolecithin and phosphatidic acid did not show any significant change by the effect of thioacetamide. In contrast, thioacetamide induced a significant decrease in the levels of phosphatidylserine, sphingomyelin, phosphatidylinositol and diphosphatidylglycerol. After 75 minutes of intraperitoneal label injection, specific radioactivity of all the above phospholipids with the exception of phosphatidylethanolamine and phosphatidylcholine significantly increased. After 13 hours of isotope administration the specific radioactivity of almost all studied phospholipid classes was elevated, except for phosphatidic acid, the specific radioactivity of which did not change and for diphosphatidylglycerol which showed a decrease in specific radioactivity. These results suggest that under thioacetamide treatment brain phospholipids undergo metabolic transformations that may contribute to the hepatic encephalopathy induced by thioacetamide.
研究了对照大鼠以及用硫代乙酰胺处理3天的大鼠的脑磷脂组成和[32P]正磷酸盐掺入脑磷脂的情况。硫代乙酰胺的作用未使脑磷脂含量、磷脂酰胆碱、磷脂酰乙醇胺、溶血卵磷脂和磷脂酸发生任何显著变化。相比之下，硫代乙酰胺导致磷脂酰丝氨酸、鞘磷脂、磷脂酰肌醇和二磷脂酰甘油水平显著降低。腹腔注射标记物75分钟后，除磷脂酰乙醇胺和磷脂酰胆碱外，上述所有磷脂的比放射性均显著增加。给予同位素13小时后，几乎所有研究的磷脂类别的比放射性均升高，但磷脂酸的比放射性未改变，二磷脂酰甘油的比放射性降低。这些结果表明，在硫代乙酰胺处理下，脑磷脂会发生代谢转变，这可能与硫代乙酰胺诱导的肝性脑病有关。
