[A new method for determination of ATPase activity in tissue homogenates].
作者信息
Martino G, Testa M, Vicinanza P, Habetswallner E
出版信息
Boll Soc Ital Biol Sper. 1978 Dec 30;54(24):2577-82.
PMID:227435
Abstract
Authors describe a new method to determine Mg2+ and Na+K+ATPase activity on extremely small samples of tissue homogenates. They use specific inhibitors to discern between the two activities and a sensitive colorimetric method to dose Pi released from ATP, Lower limit of sensitivity is about 0,0100 micromoles of Pi/mg proteins/hour.
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