College of Life Sciences, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China.
Sci China Life Sci. 2012 Jun;55(6):497-506. doi: 10.1007/s11427-012-4332-9. Epub 2012 Jun 29.
The expression of FOA1 (F-box overexpressed/oppressed ABA signaling) in different organs of Arabidopsis, and in response to ABA and NaCl, was analyzed. The expression level of FOA1 is higher in the root and is lower in the stem, and is induced rapidly by ABA and NaCl. The phenotypes of T-DNA insertion mutant foa1 and FOA1 overexpression lines FOA1ox1 and FOA1ox2 were analyzed. The foa1 mutant exhibited a lower germination rate, shorter root length, more stomatal opening, increased proline accumulation and hypersensitivity to ABA compared with the wild type. In contrast, the overexpression lines showed lower sensitivity to ABA than the wild type. The expression levels of several ABA and stress-responsive transcription factors and genes were altered in the foa1 mutant in response to ABA. Compared with the wild type, the expression levels of ABA-responsive transcription factors were higher, but ABA and stress-responsive genes were lower in foa1 mutant. This study demonstrates that FOA1 is an ABA signaling-related gene, and may play a negative role in ABA signaling.
分析了 FOA1（F-box 过度表达/抑制 ABA 信号）在拟南芥不同器官中的表达情况，以及对 ABA 和 NaCl 的反应。FOA1 的表达水平在根中较高，在茎中较低，并且被 ABA 和 NaCl 快速诱导。分析了 T-DNA 插入突变体 foa1 和 FOA1 过表达系 FOA1ox1 和 FOA1ox2 的表型。与野生型相比，foa1 突变体的萌发率较低，根长较短，气孔开度较大，脯氨酸积累增加，对 ABA 更敏感。相比之下，过表达系对 ABA 的敏感性低于野生型。FOA1 突变体在响应 ABA 时，几种 ABA 和应激响应转录因子和基因的表达水平发生了改变。与野生型相比，ABA 响应转录因子的表达水平较高，但 ABA 和应激响应基因的表达水平较低。本研究表明，FOA1 是一个与 ABA 信号相关的基因，可能在 ABA 信号转导中发挥负调控作用。
