Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education and the Key Laboratory of Non-Wood Forest Products of Forestry Ministry, Central South University of Forestry and Technology, Changsha, 410004, China.
Engineering Technology Research Center of Southern Hilly and Mountainous Ecological Non-Wood Forest Industry of Hunan Province, Changsha, 410004, China.
Planta. 2023 Aug 11;258(3):65. doi: 10.1007/s00425-023-04222-z.
Ectopic expression of Camellia oleifera Abel. gibberellin 20-oxidase 1 caused a taller phenotype, promoted secondary cell wall deposition, leaf enlargement, and early flowering, and reduced chlorophyll and anthocyanin accumulation and seed enlargement phenotype in Arabidopsis. Plant height and secondary cell wall (SCW) deposition are important plant traits. Gibberellins (GAs) play important roles in regulating plant height and SCWs deposition. Gibberellin 20-oxidase (GA20ox) is an important enzyme involved in GA biosynthesis. In the present study, we identified a GA synthesis gene in Camellia oleifera. The total length of the CoGA20ox1 gene sequence was 1146 bp, encoding 381 amino acids. Transgenic plants with CoGA20ox1 had a taller phenotype; a seed enlargement phenotype; promoted SCWs deposition, leaf enlargement, and early flowering; and reduced chlorophyll and anthocyanin accumulation. Genetic analysis showed that the mutant ga20ox1-3 Arabidopsis partially rescued the phenotype of CoGA20ox1 overexpression plants. The results showed that CoGA20ox1 participates in the growth and development of C. oleifera. The morphological changes in CoGA20ox1 overexpressed plants provide a theoretical basis for further exploration of GA biosynthesis and analysis of the molecular mechanism in C. oleifera.
油茶 20 氧化酶 1 的异位表达导致拟南芥植株增高表型，促进次生细胞壁沉积、叶片增大和早花，并减少叶绿素和花青苷积累和种子增大表型。株高和次生细胞壁（SCW）沉积是重要的植物性状。赤霉素（GAs）在调节株高和 SCW 沉积中起重要作用。赤霉素 20-氧化酶（GA20ox）是参与 GA 生物合成的重要酶。本研究在油茶中鉴定了一个 GA 合成基因。CoGA20ox1 基因全长 1146bp，编码 381 个氨基酸。转 CoGA20ox1 基因植物表现出较高的表型；种子增大表型；促进次生细胞壁沉积、叶片增大和早花；并减少叶绿素和花青苷的积累。遗传分析表明，突变体 ga20ox1-3 部分挽救了 CoGA20ox1 过表达植株的表型。结果表明，CoGA20ox1 参与了油茶的生长发育。CoGA20ox1 过表达植株的形态变化为进一步探索 GA 生物合成和分析油茶中的分子机制提供了理论依据。
