School of Pharmacy, Sungkyunkwan University, Suwon, Republic of Korea.
Am J Chin Med. 2012;40(3):467-80. doi: 10.1142/S0192415X1250036X.
This study examined the hepatoprotective effect of the HV-P411 complex, an herbal extract mixture from the seeds of Vitis vinifera, Schisandra chinensis and Taraxacum officinale, against D-galactosamine (D-GalN)-induced hepatitis. Hepatotoxicity was induced by D-GalN (700 mg/kg, i.p.), and the HV-P411 complex was administered orally 48, 24, and 2 h before and 6 h after D-GalN injection. Increases in serum aminotransferase activity and lipid peroxidation and a decrease in hepatic glutathione content were attenuated by the HV-P411 complex 24 h after D-GalN treatment. The HV-P411 complex attenuated the increases in serum tumor necrosis factor-α, interleukin (IL)-6 level and cyclooxygenase-2 protein production and their mRNA expressions, while increases in serum IL-10 level and heme oxygenase-1 protein production and their mRNA expressions were augmented by the HV-P411 complex. The increased translocation of nuclear factor-κB and c-Jun phosphorylation were attenuated by treatment with the HV-P411 complex. Our results suggest that the HV-P411 complex prevents D-GalN-induced hepatotoxicity via antioxidative and anti-inflammatory activities.
本研究考察了 HV-P411 复合物（一种来自酿酒葡萄、五味子和蒲公英种子的草药提取物混合物）对 D-半乳糖胺（D-GalN）诱导的肝炎的肝保护作用。通过 D-GalN（700mg/kg，腹腔注射）诱导肝毒性，HV-P411 复合物在 D-GalN 注射前 48、24 和 2 小时以及注射后 6 小时口服给予。HV-P411 复合物可减轻 D-GalN 处理后 24 小时血清转氨酶活性和脂质过氧化的增加以及肝谷胱甘肽含量的降低。HV-P411 复合物可减弱血清肿瘤坏死因子-α、白细胞介素（IL）-6 水平和环氧化酶-2 蛋白生成及其 mRNA 表达的增加，同时增加血清 IL-10 水平和血红素加氧酶-1 蛋白生成及其 mRNA 表达。HV-P411 复合物可减轻核因子-κB 的易位和 c-Jun 磷酸化的增加。我们的结果表明，HV-P411 复合物通过抗氧化和抗炎活性预防 D-GalN 诱导的肝毒性。
