微小 RNA-124 是侧脑室神经命运决定因子。
MicroRNA-124 is a subventricular zone neuronal fate determinant.
机构信息
Department of Experimental Medical Science, Wallenberg Neuroscience Center and Lund Stem Cell Center, Lund University, 22184 Lund, Sweden.
出版信息
J Neurosci. 2012 Jun 27;32(26):8879-89. doi: 10.1523/JNEUROSCI.0558-12.2012.
Abstract
New neurons are continuously generated from neural stem cells with astrocyte properties, which reside in close proximity to the ventricle in the postnatal and adult brain. In this study we found that microRNA-124 (miR-124) dictates postnatal neurogenesis in the mouse subventricular zone. Using a transgenic reporter mouse we show that miR-124 expression is initiated in the rapid amplifying progenitors and remains expressed in the resulting neurons. When we stably inhibited miR-124 in vivo, neurogenesis was blocked, leading to the appearance of ectopic cells with astrocyte characteristics in the olfactory bulb. Conversely, when we overexpressed miR-124, neural stem cells were not maintained in the subventricular zone and neurogenesis was lost. In summary, our results demonstrate that miR-124 is a neuronal fate determinant in the subventricular zone.
摘要
新神经元由具有星形胶质细胞特性的神经干细胞不断产生，这些细胞位于出生后和成年期大脑的脑室附近。在这项研究中，我们发现 microRNA-124（miR-124）决定了小鼠侧脑室下区的出生后神经发生。使用转基因报告小鼠，我们表明 miR-124 的表达在快速扩增祖细胞中开始，并在产生的神经元中持续表达。当我们在体内稳定抑制 miR-124 时，神经发生被阻断，导致嗅球中出现具有星形胶质细胞特征的异位细胞。相反，当我们过表达 miR-124 时，神经干细胞不在侧脑室下区维持，神经发生丢失。总之，我们的结果表明，miR-124 是侧脑室下区神经元命运的决定因素。
相似文献
1
MicroRNA-124 is a subventricular zone neuronal fate determinant.微小 RNA-124 是侧脑室神经命运决定因子。
J Neurosci. 2012 Jun 27;32(26):8879-89. doi: 10.1523/JNEUROSCI.0558-12.2012.
2
microRNA-125 distinguishes developmentally generated and adult-born olfactory bulb interneurons.miRNA-125 区分发育产生和成年新生嗅球中间神经元。
Development. 2014 Apr;141(7):1580-8. doi: 10.1242/dev.101659. Epub 2014 Mar 5.
3
Continuous postnatal neurogenesis contributes to formation of the olfactory bulb neural circuits and flexible olfactory associative learning.持续的产后神经发生有助于嗅球神经回路的形成和灵活的嗅觉联想学习。
J Neurosci. 2014 Apr 23;34(17):5788-99. doi: 10.1523/JNEUROSCI.0674-14.2014.
4
Protein expression differs between neural progenitor cells from the adult rat brain subventricular zone and olfactory bulb.成年大鼠脑室下区和嗅球的神经祖细胞之间的蛋白质表达存在差异。
BMC Neurosci. 2008 Jan 16;9:7. doi: 10.1186/1471-2202-9-7.
5
Neuronal replacement in the injured olfactory bulb.嗅球损伤后的神经元替代。
Exp Neurol. 2011 Apr;228(2):270-82. doi: 10.1016/j.expneurol.2011.01.021. Epub 2011 Feb 17.
6
Long Non-coding RNA T-UCstem1 Controls Progenitor Proliferation and Neurogenesis in the Postnatal Mouse Olfactory Bulb through Interaction with miR-9.长非编码 RNA T-UCstem1 通过与 miR-9 相互作用控制出生后小鼠嗅球祖细胞的增殖和神经发生。
Stem Cell Reports. 2020 Oct 13;15(4):836-844. doi: 10.1016/j.stemcr.2020.08.009. Epub 2020 Sep 24.
7
The olfactory conditioning in the early postnatal period stimulated neural stem/progenitor cells in the subventricular zone and increased neurogenesis in the olfactory bulb of rats.出生后早期的嗅觉条件反射刺激了大鼠脑室下区的神经干细胞/祖细胞，并增加了嗅球中的神经发生。
Neuroscience. 2008 Jan 2;151(1):120-8. doi: 10.1016/j.neuroscience.2007.07.051. Epub 2007 Aug 30.
8
Lineage analysis of quiescent regenerative stem cells in the adult brain by genetic labelling reveals spatially restricted neurogenic niches in the olfactory bulb.通过基因标记对成体大脑中静止的再生干细胞进行谱系分析，揭示了嗅球中空间受限的神经发生微环境。
Eur J Neurosci. 2009 Jul;30(1):9-24. doi: 10.1111/j.1460-9568.2009.06798.x. Epub 2009 Jun 25.
9
Analysis of neuronal proliferation, migration and differentiation in the postnatal brain using equine infectious anemia virus-based lentiviral vectors.利用马传染性贫血病毒为基础的慢病毒载体分析出生后大脑中的神经元增殖、迁移和分化。
Gene Ther. 2009 Aug;16(8):1021-33. doi: 10.1038/gt.2009.58. Epub 2009 May 28.
10
Stem cell regionalization during olfactory bulb neurogenesis depends on regulatory interactions between and .嗅球神经发生过程中的干细胞区域化依赖于 和 之间的调控相互作用。
Elife. 2020 Aug 7;9:e58215. doi: 10.7554/eLife.58215.
引用本文的文献
1
High-fat diet triggers transcriptomic changes in the olfactory bulb.高脂饮食引发嗅球的转录组变化。
Heliyon. 2025 Jan 22;11(3):e42196. doi: 10.1016/j.heliyon.2025.e42196. eCollection 2025 Feb 15.
2
miR-124 coordinates metabolic regulators acting at early stages of human neurogenesis.miR-124 协调代谢调节剂在人类神经发生早期阶段的作用。
Commun Biol. 2024 Oct 25;7(1):1393. doi: 10.1038/s42003-024-07089-2.
3
Dual-specificity protein phosphatase 6 (DUSP6) overexpression reduces amyloid load and improves memory deficits in male 5xFAD mice.双特异性蛋白磷酸酶6（DUSP6）过表达可降低雄性5xFAD小鼠的淀粉样蛋白负荷并改善记忆缺陷。
Front Aging Neurosci. 2024 Jun 28;16:1400447. doi: 10.3389/fnagi.2024.1400447. eCollection 2024.
4
Fetal Brain-Derived Exosomal miRNAs from Maternal Blood: Potential Diagnostic Biomarkers for Fetal Alcohol Spectrum Disorders (FASDs).胎儿血源性外泌体 miRNAs 作为胎儿酒精谱系障碍（FASDs）的潜在诊断生物标志物。
Int J Mol Sci. 2024 May 27;25(11):5826. doi: 10.3390/ijms25115826.
5
mC methylated lncRncr3-MeCP2 interaction restricts miR124a-initiated neurogenesis.mC 甲基化 lncRNA ncr3-MeCP2 相互作用限制 miR124a 启动的神经发生。
Nat Commun. 2024 Jun 15;15(1):5136. doi: 10.1038/s41467-024-49368-w.
6
Role of miRNAs in Brain Development.miRNAs 在大脑发育中的作用。
Microrna. 2024;13(2):96-109. doi: 10.2174/0122115366287127240322054519.
7
Optimized miR-124 reporters uncover differences in miR-124 expression among neuronal populations .优化后的miR-124报告基因揭示了神经元群体中miR-124表达的差异。
Front Neurosci. 2023 Oct 18;17:1257599. doi: 10.3389/fnins.2023.1257599. eCollection 2023.
8
MicroRNA‑124: an emerging therapeutic target in central nervous system disorders.微小 RNA-124：中枢神经系统疾病治疗的新兴靶点。
Exp Brain Res. 2023 May;241(5):1215-1226. doi: 10.1007/s00221-022-06524-2. Epub 2023 Mar 24.
9
Signature of miRNAs derived from the circulating exosomes of patients with amyotrophic lateral sclerosis.来自肌萎缩侧索硬化症患者循环外泌体的微小RNA特征
Front Aging Neurosci. 2023 Feb 10;15:1106497. doi: 10.3389/fnagi.2023.1106497. eCollection 2023.
10
Molecular Mechanisms Involved in the Regulation of Neurodevelopment by miR-124.miR-124 调控神经发育的分子机制
Mol Neurobiol. 2023 Jul;60(7):3569-3583. doi: 10.1007/s12035-023-03271-5. Epub 2023 Feb 25.
本文引用的文献
1
Capture of microRNA-bound mRNAs identifies the tumor suppressor miR-34a as a regulator of growth factor signaling.微 RNA 结合 mRNA 的捕获鉴定肿瘤抑制因子 miR-34a 作为生长因子信号的调节剂。
PLoS Genet. 2011 Nov;7(11):e1002363. doi: 10.1371/journal.pgen.1002363. Epub 2011 Nov 10.
2
Genome-wide identification of microRNA targets in human ES cells reveals a role for miR-302 in modulating BMP response.人类胚胎干细胞中 miRNA 靶基因的全基因组鉴定揭示了 miR-302 在调节 BMP 反应中的作用。
Genes Dev. 2011 Oct 15;25(20):2173-86. doi: 10.1101/gad.17221311.
3
MicroRNA profiling in subventricular zone after stroke: MiR-124a regulates proliferation of neural progenitor cells through Notch signaling pathway.中风后侧脑室下区的 microRNA 谱分析：miR-124a 通过 Notch 信号通路调节神经祖细胞的增殖。
PLoS One. 2011;6(8):e23461. doi: 10.1371/journal.pone.0023461. Epub 2011 Aug 26.
4
MicroRNAs can generate thresholds in target gene expression.微小 RNA 可以在靶基因表达中产生阈值。
Nat Genet. 2011 Aug 21;43(9):854-9. doi: 10.1038/ng.905.
5
miR-124a is required for hippocampal axogenesis and retinal cone survival through Lhx2 suppression.miR-124a 通过抑制 Lhx2 来促进海马体轴突发生和视网膜锥体存活。
Nat Neurosci. 2011 Aug 21;14(9):1125-34. doi: 10.1038/nn.2897.
6
Direct reprogramming of adult human fibroblasts to functional neurons under defined conditions.在特定条件下，直接将成体人成纤维细胞重编程为功能性神经元。
Cell Stem Cell. 2011 Aug 5;9(2):113-8. doi: 10.1016/j.stem.2011.07.002. Epub 2011 Jul 28.
7
MicroRNA-mediated conversion of human fibroblasts to neurons.微小 RNA 介导的人成纤维细胞向神经元的转化。
Nature. 2011 Jul 13;476(7359):228-31. doi: 10.1038/nature10323.
8
MicroRNA-124 promotes microglia quiescence and suppresses EAE by deactivating macrophages via the C/EBP-α-PU.1 pathway.miRNA-124 通过 C/EBP-α-PU.1 通路使小胶质细胞静息并抑制小胶质细胞活化从而抑制 EAE。
Nat Med. 2011 Jan;17(1):64-70. doi: 10.1038/nm.2266. Epub 2010 Dec 5.
9
LNA-FISH for detection of microRNAs in frozen sections.用于在冰冻切片中检测微小RNA的锁核酸荧光原位杂交技术
Methods Mol Biol. 2010;659:165-71. doi: 10.1007/978-1-60761-789-1_11.
10
Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors.使用 microRNA 调控的慢病毒载体追踪多能培养中的神经前体细胞分化。
Proc Natl Acad Sci U S A. 2010 Jun 22;107(25):11602-7. doi: 10.1073/pnas.1006568107. Epub 2010 Jun 7.
Zotero 插件