在简化的无蛋白质DNA侧向流动分析中固定和图案化DNA
Immobilizing and Patterning DNA on Simplified Protein-Free DNA-Based Lateral Flow Assays.
作者信息
Lee Hyunbin, Hwang Haesoo, Lee Ji Young, Lee Min-Young, Kim Youngeun
机构信息
Department of Materials Science and Engineering, College of Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.
Research Institute of Advanced Materials, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.
出版信息
ACS Omega. 2025 Mar 31;10(14):14115-14120. doi: 10.1021/acsomega.4c11255. eCollection 2025 Apr 15.
This work presents a comprehensive study on DNA-based LFAs that completely avoids the use of proteins or pretreatment buffer. We address key fundamental challenges including optimizing buffer conditions for effective DNA immobilization on paper, understanding sequence-dependent detection, and ultimately developing patterning techniques that allow DNA patterns to appear on paper that exhibit distinct shapes depending on the introduced input sequences.
这项工作对基于DNA的侧向流动分析装置进行了全面研究，完全避免了使用蛋白质或预处理缓冲液。我们解决了关键的基础挑战，包括优化缓冲条件以实现DNA在纸上的有效固定、理解序列依赖性检测，并最终开发出图案化技术，使DNA图案能出现在纸上，这些图案根据引入的输入序列呈现出不同的形状。
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