Institute of Molecular Biosciences, Massey University, Private Bag 11222, Palmerston North, New Zealand.
FEBS Lett. 2012 Jul 30;586(16):2563-9. doi: 10.1016/j.febslet.2012.06.035. Epub 2012 Jun 29.
Epichloë festucae Fl1 in association with Lolium perenne synthesizes a diverse range of indole-diterpene bioprotective metabolites, including lolitrem B, a potent tremorgen. The ltm genes responsible for the synthesis of these metabolites are organized in three clusters at a single sub-telomeric locus in the genome of E. festucae. Here we resolve the genetic basis for the remarkable indole-diterpene diversity observed in planta by analyzing products that accumulate in associations containing ltm deletion mutants of E. festucae and in cells of Penicillium paxilli containing copies of these genes under the control of a P. paxilli biosynthetic gene promoter. We propose a biosynthetic scheme to account for this metabolic diversity.
内生真菌弗氏新棒束孢 Fl1 与多年生黑麦草共生合成了一系列不同的吲哚二萜类生物保护代谢物，包括洛替雷姆 B，一种有效的震颤原。负责这些代谢物合成的 ltm 基因在弗氏新棒束孢基因组的单个亚端粒位点上以三个簇的形式组织。在这里，我们通过分析在含有弗氏新棒束孢 ltm 缺失突变体的共生体中积累的产物以及在含有这些基因拷贝的青霉菌细胞中分析来解析植物中观察到的吲哚二萜类化合物的显著多样性的遗传基础，这些基因在青霉菌生物合成基因启动子的控制下。我们提出了一个生物合成方案来解释这种代谢多样性。
