Department of Biological Sciences, Lehigh University, Bethlehem, PA 18015, USA.
FEBS Lett. 2012 Jul 30;586(16):2313-7. doi: 10.1016/j.febslet.2012.06.037. Epub 2012 Jun 29.
The selective deamination of adenosines (A) to inosines (I) in messenger RNAs (mRNAs) can alter the encoded protein's amino acid sequence, with often critical consequences on protein stability, localization, and/or function. Insulin-like growth factor-binding protein 7 (IGFBP7) supports cell-adhesion and stimulates fibroblast proliferation with IGF and insulin. It exists in both proteolytically processed and unprocessed forms with altered cell-extracellular matrix interactions. Here we show that editing of IGFBP7 transcripts impacts the protein's susceptibility to proteolytic cleavage, thus providing a means for a cell to modulate its functionality through A-to-I RNA editing.
腺嘌呤（A）在信使 RNA（mRNA）中选择性脱氨为肌苷（I），可以改变编码蛋白的氨基酸序列，对蛋白质稳定性、定位和/或功能通常有重要影响。胰岛素样生长因子结合蛋白 7（IGFBP7）与胰岛素和 IGF 一起支持细胞黏附和刺激成纤维细胞增殖。它以两种形式存在，一种是经过蛋白水解处理的形式，另一种是未经处理的形式，改变了细胞与细胞外基质的相互作用。在这里，我们表明 IGFBP7 转录本的编辑会影响蛋白质对蛋白水解切割的敏感性，从而为细胞提供了一种通过 A 到 I 的 RNA 编辑来调节其功能的方法。
