Department of Molecular Biology, University of Salzburg, 5020 Salzburg, Austria.
J Control Release. 2012 Sep 10;162(2):391-9. doi: 10.1016/j.jconrel.2012.06.031. Epub 2012 Jun 29.
Driven by constantly increasing knowledge about skin immunology, vaccine delivery via the cutaneous route has recently gained renewed interest. Considering its richness in immunocompetent cells, targeting antigens to the skin is considered to be more effective than intramuscular or subcutaneous injections. However, circumvention of the superficial layer of the skin, the stratum corneum, represents the major challenge for cutaneous immunization. An optimal delivery method has to be effective and reliable, but also highly adaptable to specific demands, should avoid the use of hypodermic needles and the requirement of specially trained healthcare workers. The P.L.E.A.S.E. (Precise Laser Epidermal System) device employed in this study for creation of aqueous micropores in the skin fulfills these prerequisites by combining the precision of its laser scanning technology with the flexibility to vary the number, density and the depth of the micropores in a user-friendly manner. We investigated the potential of transcutaneous immunization via laser-generated micropores for induction of specific immune responses and compared the outcomes to conventional subcutaneous injection. By targeting different layers of the skin we were able to bias polarization of T cells, which could be modulated by addition of adjuvants. The P.L.E.A.S.E. device represents a highly effective and versatile platform for transcutaneous vaccination.
受皮肤免疫学知识不断增长的推动，通过皮肤途径输送疫苗最近重新引起了人们的兴趣。考虑到皮肤中免疫细胞丰富，将抗原靶向皮肤被认为比肌肉内或皮下注射更有效。然而，绕过皮肤的浅层，即角质层，是皮肤免疫接种的主要挑战。一种最佳的输送方法必须既有效又可靠，但也要高度适应特定的需求，应避免使用皮下注射针和特别训练有素的医疗保健工作者的要求。本研究中用于在皮肤中创建水性微孔的 P.L.E.A.S.E.（精确激光表皮系统）设备通过将其激光扫描技术的精度与以用户友好的方式改变微孔的数量、密度和深度的灵活性相结合，满足了这些要求。我们研究了通过激光产生的微孔进行经皮免疫接种诱导特异性免疫应答的潜力，并将结果与传统的皮下注射进行了比较。通过靶向皮肤的不同层，我们能够使 T 细胞发生偏极化，并且可以通过添加佐剂来调节这种偏极化。P.L.E.A.S.E. 设备是一种高效、多功能的经皮疫苗接种平台。
