Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Granada, Spain.
Plant Signal Behav. 2012 Jul;7(7):779-81. doi: 10.4161/psb.20570. Epub 2012 Jul 1.
Reactive oxygen species and NO are involved in the signaling pathway of programmed cell death (PCD). Information concerning the role of these molecules in self-incompatible pollination is scarce especially in non-model species studied in vivo. We recently reported that in the olive tree, compatible and self-incompatible pollen have different levels of reactive oxygen and nitrogen species and that PCD is induced in self-incompatible pollen. Levels of O 2 (.-) and NO are higher in pollen after self-incompatible pollination than after compatible pollination. The presence of these reactive species was concomitant with the presence of peroxynitrite. Similar results were obtained on pollen-germination experiments both in vivo and in vitro. These data, together with observations made after treating pollinated flowers with scavengers, suggest that peroxynitrite plays a role in PCD induced after self-incompatible pollination and we propose here a model to describe the way in which it might work.
活性氧和一氧化氮参与细胞程序性死亡（PCD）的信号通路。关于这些分子在自交不亲和授粉中的作用的信息很少，特别是在体内研究的非模式物种中。我们最近报道，在橄榄树中，亲和和自交不亲和花粉具有不同水平的活性氧和氮物种，并且自交不亲和花粉中诱导了 PCD。自交不亲和授粉后花粉中的 O 2 (-)和 NO 水平高于亲和授粉后。这些活性物质的存在伴随着过氧亚硝酸盐的存在。在体内和体外的花粉萌发实验中都得到了类似的结果。这些数据，以及用清除剂处理授粉花朵后的观察结果表明，过氧亚硝酸盐在自交不亲和授粉后诱导的 PCD 中起作用，我们在这里提出了一个模型来描述它的作用方式。
