Malerba Massimo, Cerana Raffaella
Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy.
Dipartimento di Scienze dell'Ambiente e della Terra, Università degli Studi di Milano-Bicocca, Milan, Italy.
Protoplasma. 2018 Jul;255(4):1079-1087. doi: 10.1007/s00709-017-1200-2. Epub 2018 Feb 6.
Temperatures above the optimum are sensed as heat stress (HS) by all living organisms and represent one of the major environmental challenges for plants. Plants can cope with HS by activating specific defense mechanisms to minimize damage and ensure cellular functionality. One of the most common effects of HS is the overproduction of reactive oxygen and nitrogen species (ROS and RNS). The role of ROS and RNS in the regulation of many plant physiological processes is well established. On the contrary, in plants very little is known about the physiological role of peroxynitrite (ONOO), the RNS species generated by the interaction between NO and O. In this work, the role of ONOO on some of the stress responses induced by HS in tobacco BY-2 cultured cells has been investigated by measuring these responses both in the presence and in the absence of 2,6,8-trihydroxypurine (urate), a specific scavenger of ONOO. The obtained results suggest a potential role for ONOO in some of the responses induced by HS in tobacco cultured cells. In particular, ONOO seems implicated in a form of cell death showing apoptotic features and in the regulation of the levels of proteins involved in the response to stress.
所有生物都会将高于最适温度的温度感知为热胁迫（HS），这是植物面临的主要环境挑战之一。植物可以通过激活特定的防御机制来应对热胁迫，以尽量减少损害并确保细胞功能。热胁迫最常见的影响之一是活性氧和氮物种（ROS和RNS）的过量产生。ROS和RNS在许多植物生理过程调控中的作用已得到充分证实。相反，在植物中，关于过氧亚硝酸盐（ONOO）的生理作用知之甚少，ONOO是由NO和O相互作用产生的RNS物种。在这项工作中，通过在存在和不存在2,6,8 - 三羟基嘌呤（尿酸盐）（一种ONOO的特异性清除剂）的情况下测量这些反应，研究了ONOO对烟草BY - 2培养细胞中热胁迫诱导的一些应激反应的作用。所得结果表明ONOO在烟草培养细胞中热胁迫诱导的一些反应中具有潜在作用。特别是，ONOO似乎与一种具有凋亡特征的细胞死亡形式以及对应激反应相关蛋白质水平的调节有关。
