基于质谱的核苷酸、核苷和碱基分析——在饲料补充剂中的应用。

Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases--application to feed supplements.

机构信息

Department of Chemistry, Division of Analytical Chemistry, University of Natural Resources and Life Sciences-BOKU, Vienna, Austria.

出版信息

Anal Bioanal Chem. 2012 Aug;404(3):799-808. doi: 10.1007/s00216-012-6170-9. Epub 2012 Jun 30.

DOI:10.1007/s00216-012-6170-9

PMID:22752403

Abstract

In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100% aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L(-1) could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.

摘要

本工作提出了一种精确的基于 MS 的方法，用于定量分析作为动物饲料添加剂的酵母提取物中的核苷酸、核苷和碱基。与亲水相互作用色谱或离子对反相分离相比，采用与 100%水相流动相兼容的固定相的反相色谱得到了更好的分析性能。这种新的分离方法与分子和元素质谱相结合。通过使用 RP-LC-MS-MS，可以获得所有研究化合物的出色检测限 <1 μmol L(-1)。元素形态分析方法可以通过磷检测来测定核苷酸。LC-ICP-MS 的灵敏度比 LC-MS-MS 低 1-2 个数量级。使用互补的 MS 检测对酵母产品进行定量分析，得到了一致的结果。