Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan.
Mol Biotechnol. 2013 Jun;54(2):320-30. doi: 10.1007/s12033-012-9575-y.
The performance of tree species is influenced by environmental factors and growth stages. To evaluate the practical performance of transgenic tree species, it is insufficient to grow small, young trees under controlled conditions, such as in a growth chamber. Three transgenic Eucalyptus globulus lines, carrying the choline oxidase gene, were investigated for their salt tolerance and expression of the transgene at the young plantlet stage in a special netted-house. To clarify the characteristics at the young as well during the later stages, salt tolerance and the properties of the transgenic lines at large juvenile and adult stages were evaluated in the special netted-house. All transgenic lines showed high glycinebetaine content, particularly in young leaves. Trees of the transgenic line 107-1 showed low damage because of salinity stress based on the results from the chlorophyll analysis and malondialdehyde content, and they survived the high-salt-shock treatment at the large juvenile and adult stages. Only this line showed salt tolerance at all stages in the special netted-house. In this evaluation in the special netted-house, the tolerant line among young plantlets might perform better at all stages. Since evaluation in these special netted-house mimics field evaluation, line 107-1 is a potential tolerant line.
树种的表现受环境因素和生长阶段的影响。为了评估转基因树种的实际表现，仅仅在受控条件下（例如在生长室中）种植小型、年轻树木是不够的。本研究调查了携带胆碱氧化酶基因的 3 个转基因蓝桉品系在特殊网棚中的幼树阶段的耐盐性和转基因的表达。为了阐明幼树阶段和后期的特征，在特殊网棚中评估了耐盐性和大型幼树和成年阶段转基因品系的特性。所有转基因品系的甘氨酸甜菜碱含量都很高，特别是在幼叶中。根据叶绿素分析和丙二醛含量的结果，转基因品系 107-1 的树木因盐胁迫造成的损伤较低，并且在大型幼树和成年阶段的高盐冲击处理中存活下来。只有这条品系在特殊网棚的所有阶段都表现出耐盐性。在特殊网棚中的这种评估中，幼树阶段耐盐的品系在所有阶段可能表现更好。由于特殊网棚中的评估模拟了田间评估，因此品系 107-1 是一种潜在的耐盐品系。
