使用活性染料和共聚焦显微镜对切除组织进行离体成像。
Ex vivo imaging of excised tissue using vital dyes and confocal microscopy.
作者信息
Johnson Simon, Rabinovitch Peter
机构信息
University of Washington, Seattle, Washington, USA.
出版信息
Curr Protoc Cytom. 2012 Jul;Chapter 9:Unit 9.39. doi: 10.1002/0471142956.cy0939s61.
Abstract
Vital dyes routinely used for staining cultured cells can also be used to stain and image live tissue slices ex vivo. Staining tissue with vital dyes allows researchers to collect structural and functional data simultaneously and can be used for qualitative or quantitative fluorescent image collection. The protocols presented here are useful for structural and functional analysis of viable properties of cells in intact tissue slices, allowing for the collection of data in a structurally relevant environment. With these protocols, vital dyes can be applied as a research tool to disease processes and properties of tissue not amenable to cell culture-based studies.
摘要
常用于对培养细胞进行染色的活性染料也可用于对离体的活组织切片进行染色和成像。用活性染料对组织进行染色可使研究人员同时收集结构和功能数据，并且可用于定性或定量荧光图像采集。本文介绍的实验方案对于完整组织切片中细胞活性特性的结构和功能分析很有用，能够在与结构相关的环境中收集数据。通过这些实验方案，活性染料可用作一种研究工具，应用于疾病过程以及不适用于基于细胞培养研究的组织特性研究。
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