高效阳离子乙基磷酰胆碱 siRNA 载体抑制修饰乳腺癌细胞中的 GFP。
Highly efficient cationic ethylphosphatidylcholine siRNA carrier for GFP suppression in modified breast cancer cells.
机构信息
Ohio State University College of Pharmacy, 517 Parks Hall, 500 W 12th Ave, Columbus, OH 43210, USA.
出版信息
Anticancer Res. 2012 Jul;32(7):2563-6.
AIM
Cationic ethylphosphatidylcholines (ePCs) were evaluated for the delivery of siRNA in modified breast cancer cells.
MATERIALS AND METHODS
Dimyristoleoyl-ePC (C14), dioleoyl-ePC (C18), and dilauroyl-ePC (C12) nanoparticles were complexed with siRNA for green fluorescent protein (GFP) suppression in modified MCF-7 breast cancer cells. The kinetics of GFP suppression were followed over the course of 72 hours.
RESULTS
C14, which has been previously found to be particularly effective in gene transfection into primary human umbilical artery endothelial cells, was also remarkably effective as siRNA carrier, with an efficacy exceeding that of Lipofectamine RNAiMAX. The C14 toxicity remained comparable to that of RNAiMAX. The efficacy of the other tested cationic ePC formulations was less than that of C14 and RNAiMAX.
CONCLUSION
The cationic lipid C14 is a highly efficient siRNA carrier that could be used for the development of new formulations for siRNA delivery into cancer cells. A valuable advantage of the C14 formulations is the fact that they are simple, and do not require adjuvants or complex preparation procedures.
目的
评估阳离子乙基磷脂酰胆碱（ePC）在修饰乳腺癌细胞中递送 siRNA 的效果。
材料与方法
将二肉豆蔻酰基-ePC（C14）、二油酰基-ePC（C18）和二月桂酰基-ePC（C12）纳米颗粒与 siRNA 复合，以抑制修饰的 MCF-7 乳腺癌细胞中的绿色荧光蛋白（GFP）。在 72 小时的过程中跟踪 GFP 抑制的动力学。
结果
先前发现 C14 在原代人脐动脉内皮细胞中的基因转染特别有效，它作为 siRNA 载体也非常有效，其功效超过 Lipofectamine RNAiMAX。C14 的毒性仍与 RNAiMAX 相当。其他测试的阳离子 ePC 制剂的功效低于 C14 和 RNAiMAX。
结论
阳离子脂质 C14 是一种高效的 siRNA 载体，可用于开发新的用于将 siRNA 递送至癌细胞的制剂。C14 制剂的一个有价值的优势是它们简单，不需要佐剂或复杂的制备程序。
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