Cellular and Molecular Research Center, Faculty of Medecine, Guilan University Complex, Rasht 41996-13769, Iran.
Stem Cells Int. 2012;2012:369417. doi: 10.1155/2012/369417. Epub 2012 Jun 14.
The present study aims to evaluate the effect of bone marrow mesenchymal stem cells (MSCs) grafts on cognition deficit in chemically and age-induced Alzheimer's models of rats. In the first experiments aged animals (30 months) were tested in Morris water maze (MWM) and divided into two groups: impaired memory and unimpaired memory. Impaired groups were divided into two groups and cannulated bilaterally at the CA1 of the hippocampus for delivery of mesenchymal stem cells (500 × 10(3)/μL) and PBS (phosphate buffer saline). In the second experiment, Ibotenic acid (Ibo) was injected bilaterally into the nucleus basalis magnocellularis (NBM) of young rats (3 months) and animals were tested in MWM. Then, animals with memory impairment received the following treatments: MSCs (500 × 10(3)/μL) and PBS. Two months after the treatments, cognitive recovery was assessed by MWM in relearning paradigm in both experiments. Results showed that MSCs treatment significantly increased learning ability and memory in both age- and Ibo-induced memory impairment. Adult bone marrow mesenchymal stem cells show promise in treating cognitive decline associated with aging and NBM lesions.
本研究旨在评估骨髓间充质干细胞（MSCs）移植对化学诱导和年龄诱导的大鼠阿尔茨海默病模型认知缺陷的影响。在第一个实验中，对老年动物（30 个月）进行了 Morris 水迷宫（MWM）测试，并分为两组：记忆受损组和未受损组。受损组再分为两组，在海马 CA1 双侧进行 MSC（500×10^3/μL）和 PBS（磷酸盐缓冲盐水）的移植。在第二个实验中，将 Ibotenic 酸（Ibo）双侧注入大鼠基底神经核（NBM），并在 MWM 中进行动物测试。然后，对记忆受损的动物进行以下治疗：MSC（500×10^3/μL）和 PBS。治疗两个月后，通过 MWM 在两个实验中的重新学习范式评估认知恢复情况。结果表明，MSC 治疗可显著提高年龄和 Ibo 诱导的记忆损伤模型的学习能力和记忆能力。成体骨髓间充质干细胞有望治疗与衰老和 NBM 损伤相关的认知能力下降。
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