Parnow Abdolhossein, Gharakhanlou Reza, Gorginkaraji Zeinab, Rajabi Somayeh, Eslami Rasoul, Hedayati Mahdi, Mahdian Reza
Exercise Physiology Department, School of Physical Education, Razi University of Kermanshah, P.O. Box 6714414874, Kermanshah, Iran.
Int J Pept. 2012;2012:962651. doi: 10.1155/2012/962651. Epub 2012 Jun 18.
The aim of this study was to investigate effects of endurance and resistance training (ET and RT) on CGRP and AChRs at slow and fast twitch muscles and sciatic nerve in rats. Twenty-five male rats were randomly assigned into three groups including sedentary (SED), endurance training (ET), and resistance training (RT). Animals of ET exercised for 12 weeks, five times/week, and 60 min/day at 30 m/min. Animals of RT were housed in metal cage with 2 m high wire-mesh tower, with water bottles set at the top. 48 h after the last session of training protocol, animals were anaesthetized. The right sciatic nerves were removed; then, Soleus (SOL) and Tibialis anterior (TA) muscles were excised and immediately snap frozen in liquid nitrogen. All frozen tissues were stored at -80°C. Results showed that, after both ET and RT, CGRP content as well as AChR content of SOL and TA muscles significantly increased. But there was no significant difference among groups at sciatic nerve' CGRP content. In conclusion, data demonstrate that ET and RT lead to changes of CGRP and AChR content of ST and FT muscles. The changes indicate to the importance of neuromuscular activity.
本研究旨在探讨耐力训练和抗阻训练（ET和RT）对大鼠慢、快肌及坐骨神经中降钙素基因相关肽（CGRP）和乙酰胆碱受体（AChRs）的影响。25只雄性大鼠被随机分为三组，包括久坐组（SED）、耐力训练组（ET）和抗阻训练组（RT）。ET组动物进行12周训练，每周5次，每天以30米/分钟的速度运动60分钟。RT组动物饲养在带有2米高金属丝网塔的金属笼中，水瓶设置在顶部。在最后一次训练方案结束48小时后，对动物进行麻醉。取出右侧坐骨神经；然后，切除比目鱼肌（SOL）和胫骨前肌（TA），并立即在液氮中速冻。所有冷冻组织均储存在-80°C。结果显示，ET和RT后，SOL和TA肌肉的CGRP含量以及AChR含量均显著增加。但坐骨神经CGRP含量在各组间无显著差异。总之，数据表明ET和RT导致慢、快肌CGRP和AChR含量发生变化。这些变化表明神经肌肉活动的重要性。
