Garcon N M, Groothuis J, Brown S, Lauer B, Pietrobon P, Six H R
Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas.
Antiviral Res. 1990 Aug;14(2):109-16. doi: 10.1016/0166-3542(90)90048-c.
To ascertain whether live attenuated or inactivated vaccines can be considered equivalent, we examined the primary antibody response of children following vaccination with influenza virus antigens in three different formulations. Nine children received cold recombinant vaccine (CRV) containing A/Korea/82 (H3N2) and A/Dunedin/83 (H1N1) variants. Eight of these children responded to HA of the H3N2 subtype and the major portion of the elicited antibody was in the IgG1 subclass. Antibody of low titer in the IgG2 and IgG3 subclasses was detected in two and six serum specimens, respectively. Six of the nine children administered with CRV responded to the H1 antigen and only IgG1 antibody was detected. Serum specimens from eight children less than one year of age (5 less than 6 months of age) who had developed an antibody response to trivalent inactivated vaccine (TIV) vaccination were examined. High levels of IgG1 antibody to purified H3 were detected in all eight children. Low titers of antibody in IgG2 and IgG3 subclasses were detected in two and five children, respectively. Antibody responses to purified H1 showed a similar subclass distribution. In order to examine secondary response, eight children primed by immunization with TIV vaccine were subsequently given a single booster dose of purified hemagglutinin (HA) conjugated to diphtheria toxoid (HA-D). In 6/8 specimens antibody rises were detected to purified H3 and H1 antigens. Prior to the HA-D immunization, low levels of HA specific IgG1 antibody were detected in all serum specimens and vaccine induced responses were primarily of the IgG1 subclass.(ABSTRACT TRUNCATED AT 250 WORDS)
为确定减毒活疫苗或灭活疫苗是否可被视为等效，我们检测了三种不同剂型流感病毒抗原疫苗接种后儿童的初次抗体反应。9名儿童接种了含有A/韩国/82（H3N2）和A/达尼丁/83（H1N1）变异株的冷重组疫苗（CRV）。其中8名儿童对H3N2亚型的血凝素（HA）产生反应，所诱导抗体的主要部分为IgG1亚类。分别在2份和6份血清标本中检测到IgG2和IgG3亚类的低滴度抗体。接种CRV的9名儿童中有6名对H1抗原产生反应，仅检测到IgG1抗体。检测了8名对三价灭活疫苗（TIV）接种产生抗体反应的1岁以下儿童（5名小于6个月）的血清标本。所有8名儿童均检测到针对纯化H3的高水平IgG1抗体。分别在2名和5名儿童中检测到IgG2和IgG3亚类的低滴度抗体。对纯化H1的抗体反应显示出类似的亚类分布。为检测二次反应，8名经TIV疫苗免疫致敏的儿童随后接受了一剂与白喉类毒素偶联的纯化血凝素（HA-D）加强免疫。在6/8份标本中检测到针对纯化H3和H1抗原的抗体升高。在HA-D免疫前，所有血清标本中均检测到低水平的HA特异性IgG1抗体，疫苗诱导的反应主要为IgG1亚类。（摘要截短于250字）
