Clausen M, Leier G, Witte I
Universität Oldenburg, Fachbereich Biologie, Federal Republic of Germany.
Arch Toxicol. 1990;64(6):497-501. doi: 10.1007/BF01977633.
U 46 D Fluid (the dimethylammonium salt of 2,4-dichlorophenoxyacetic acid in a commercial formulation) was more toxic to human fibroblasts than 2,4-dichlorophenoxyacetic acid (2,4-D). Moreover, U 46 D Fluid induced single-strand breaks at apurinic/apyrimidinic (AP) sites of heat-acid treated PM2 DNA while 2,4-D did not. The 1H-NMR spectrum of the dimethylammonium (DMA) salt of 2,4-D indicated the formation of a complex via a six-membered aggregate of the DMA ion and the acid group of 2,4-D. The role of complex formation of the DMA salt of 2,4-D as a cause of single-strand breaking activity and increased cytotoxicity is discussed.
U 46 D液剂（市售配方中2,4-二氯苯氧乙酸的二甲铵盐）对人成纤维细胞的毒性比2,4-二氯苯氧乙酸（2,4-D）更大。此外，U 46 D液剂在热酸处理的PM2 DNA的无嘌呤/无嘧啶（AP）位点诱导单链断裂，而2,4-D则不会。2,4-D的二甲铵（DMA）盐的1H-NMR光谱表明，通过DMA离子与2,4-D的酸基团形成六元聚集体形成了一种复合物。本文讨论了2,4-D的DMA盐形成复合物作为单链断裂活性和细胞毒性增加原因的作用。
