U46 D液（2,4-二氯苯氧乙酸二甲铵盐）与氯化铜对人成纤维细胞细胞毒性及DNA修复的协同作用

Synergistic effects of U46 D fluid (dimethylammonium salt of 2,4-D) and CuCl2 on cytotoxicity and DNA repair in human fibroblasts.

作者信息

Jacobi H, Witte I

机构信息

Department of Biology, University of Oldenburg, F.R.G.

出版信息

Toxicol Lett. 1991 Oct;58(2):159-67. doi: 10.1016/0378-4274(91)90170-b.

DOI:10.1016/0378-4274(91)90170-b

PMID:1949075

Abstract

The cytotoxicity of U46 D Fluid was tested in human fibroblasts after pretreatment with non-toxic or slightly toxic concentrations of CuCl2. While cell survival, colony-forming ability and protein synthesis were not affected by pretreatment with CuCl2, the inhibition of cell growth was enhanced as was inhibition of DNA synthesis. Synergistic effects of CuCl2 and U46 D Fluid were also detected on the induction of DNA repair measured by unscheduled DNA synthesis. While neither U46 D Fluid nor CuCl2 alone induced DNA repair, preincubation with CuCl2 followed by treatment with U46 D Fluid strongly provoked DNA repair.

摘要

在用无毒或低毒浓度的氯化铜预处理后，对人成纤维细胞进行了U46 D液的细胞毒性测试。虽然用氯化铜预处理不影响细胞存活、集落形成能力和蛋白质合成，但细胞生长抑制增强，DNA合成抑制也增强。通过非定标DNA合成测定，还检测到氯化铜和U46 D液对DNA修复诱导的协同作用。虽然单独使用U46 D液或氯化铜都不会诱导DNA修复，但先用氯化铜预孵育，然后用U46 D液处理，则会强烈激发DNA修复。