鉴定大肠杆菌K12中赋予UGA抑制活性的prfB基因突变。

Identification of the mutations in the prfB gene of Escherichia coli K12, which confer UGA suppressor activity.

作者信息

Wu E D, Inokuchi H, Ozeki H

机构信息

Department of Biophysics, Faculty of Science, Kyoto University, Japan.

出版信息

Jpn J Genet. 1990 Jun;65(3):115-9. doi: 10.1266/jjg.65.115.

DOI:10.1266/jjg.65.115

PMID:2275732

Abstract

By DNA sequencing and gene dissection, it has been revealed that Su+UGA#11, the mutant prfB of E. coli (Chang et al., 1990) has a double mutation compared with the wild-type LS653: one is a base substitution from T to C at the codon 63 and the other is from G to A at the codon 79. Both mutations cause amino acid substitution, Leu63----Phe63 (L63F) and Asp79----Gly79 (D79G), and are necessary to confer the efficient UGA suppressor activity.

摘要

通过DNA测序和基因剖析发现，大肠杆菌的突变型prfB即Su + UGA#11（Chang等人，1990年）与野生型LS653相比有双重突变：一个是密码子63处从T到C的碱基替换，另一个是密码子79处从G到A的碱基替换。这两个突变均导致氨基酸替换，即Leu63----Phe63（L63F）和Asp79----Gly79（D79G），并且对于赋予高效的UGA抑制活性是必需的。