Mathur R L, Sahai P
Ocular Biochemistry Section, Rajendra Prasad Centre for Ophthalmic Sciences, AIIMS, Ansari Nagar, New Delhi.
Lens Eye Toxic Res. 1990;7(2):143-60.
Rats maintained on a tryptophan supplemented diet and exposed to U.V. radiation showed decreased concentration of ascorbic acid in serum. In the lens, a small increase in the urea-mercaptoethanol soluble fraction was observed suggesting some oxidation of P-SH groups. The decreased concentrations of lens glutathione and ascorbic acid were accompanied with increased concentration of malondialdehyde suggesting increased oxidative stress. The activities of glutathione peroxidase decreased by about 40%. Though the activity of glutathione reductase decreased by about 58%, addition of FAD in the enzyme assay system showed restoration of lost activity. Additive effect of raised serum tryptophan concentration and ultraviolet radiation in causing damage to the eye lens is suggested.
食用补充色氨酸饮食并暴露于紫外线辐射下的大鼠，血清中抗坏血酸浓度降低。在晶状体中，观察到尿素 - 巯基乙醇可溶部分略有增加，表明部分P-SH基团发生氧化。晶状体谷胱甘肽和抗坏血酸浓度降低，同时丙二醛浓度增加，表明氧化应激增加。谷胱甘肽过氧化物酶的活性降低了约40%。虽然谷胱甘肽还原酶的活性降低了约58%，但在酶测定系统中添加FAD可使丧失的活性恢复。提示血清色氨酸浓度升高和紫外线辐射对眼晶状体造成损伤具有累加效应。
