Department of Pharmacology and Physiology, University of Zaragoza, Spain.
Int J Mol Sci. 2010 Jan 21;11(1):312-28. doi: 10.3390/ijms11010312.
Since biological membranes are composed of lipids and proteins we tested the in vitro antioxidant properties of several indoleamines from the tryptophan metabolic pathway in the pineal gland against oxidative damage to lipids and proteins of synaptosomes isolated from the rat brain. Free radicals were generated by incubation with 0.1 mM FeCl(3), and 0.1 mM ascorbic acid. Levels of malondialdehyde (MDA) plus 4-hydroxyalkenal (4-HDA), and carbonyl content in the proteins were measured as indices of oxidative damage to lipids and proteins, respectively. Pinoline was the most powerful antioxidant evaluated, with melatonin, N-acetylserotonin, 5-hydroxytryptophan, 5-methoxytryptamine, 5-methoxytryptophol, and tryptoline also acting as antioxidants.
由于生物膜是由脂质和蛋白质组成的，我们测试了来自松果腺色氨酸代谢途径的几种吲哚胺对大鼠脑突触体中脂质和蛋白质氧化损伤的体外抗氧化特性。自由基通过与 0.1 mM FeCl(3)和 0.1 mM 抗坏血酸孵育产生。丙二醛（MDA）加 4-羟烯醛（4-HDA）的水平和蛋白质中的羰基含量分别作为脂质和蛋白质氧化损伤的指标进行测量。Pinoline 是评估的最有效的抗氧化剂，褪黑素、N-乙酰血清素、5-羟色氨酸、5-甲氧基色胺、5-甲氧基色醇和色醇也具有抗氧化作用。
