Dosler Sibel, Gerceker A Alev
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Istanbul University, Turkey.
J Chemother. 2012 Jun;24(3):137-43. doi: 10.1179/1973947812Y.0000000007.
The In vitro activities of two antimicrobial cationic peptides, melittin and nisin alone and in combination with frequently used antibiotics (daptomycin, vancomycin, linezolid, ampicillin, and erythromycin), were assessed against clinical isolates of methicillin-susceptible Staphylococcus aureus, methicillin-resistant S. aureus and Enterococcus faecalis. Using the broth microdilution method, minimum inhibitory concentration (MIC) ranges of melittin and nisin against all strains were 2-8 μg/ml and 2-32 μg/ml respectively. In combination studies performed with the microdilution checkerboard method using a fractional inhibitory concentration index of ≤ 0.5 as borderline, synergistic interactions occurred more frequently with nisin-ampicillin combination against MSSA and nisin-daptomycin combination against E. faecalis strains. The results of the time-killing curve analysis demonstrated that the concentration dependent rapid bactericidal activity of nisin, and that synergism or early synergism was detected in most strains when nisin or melittin was used in combination with antibiotics even at concentrations of 0.5 × MIC.
评估了两种抗菌阳离子肽（蜂毒素和乳链菌肽）单独以及与常用抗生素（达托霉素、万古霉素、利奈唑胺、氨苄西林和红霉素）联合使用时，对甲氧西林敏感金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌和粪肠球菌临床分离株的体外活性。采用肉汤微量稀释法，蜂毒素和乳链菌肽对所有菌株的最低抑菌浓度（MIC）范围分别为2 - 8μg/ml和2 - 32μg/ml。在使用微量稀释棋盘法进行的联合研究中，以≤0.5的分数抑菌浓度指数作为临界值，乳链菌肽 - 氨苄西林联合对甲氧西林敏感金黄色葡萄球菌以及乳链菌肽 - 达托霉素联合对粪肠球菌菌株出现协同相互作用的频率更高。时间 - 杀菌曲线分析结果表明，乳链菌肽具有浓度依赖性快速杀菌活性，并且当乳链菌肽或蜂毒素与抗生素联合使用时，即使在0.5×MIC浓度下，大多数菌株中也检测到协同作用或早期协同作用。
